Anti-Proprotein Convertase 9 (PCSK9) Rabbit Polyclonal Antibody for WB, IP, IF/ICC, IHC-P, ELISA - Q8NBP7

Core Product Specifications and Parameters

Biological Background: PCSK9 Function and Localization

• Proprotein convertase subtilisin/kexin type 9 (PCSK9), also known as neural apoptosis-regulated convertase 1 (NARC-1) or PC9, is a serine protease involved in cholesterol metabolism.
• Binds to low-density lipoprotein receptor family members (LDLR, VLDLR, LRP1/APOER, LRP8/APOER2), promoting their degradation in intracellular acidic compartments. Related references: PMID:18039658
• Enhances hepatic LDLR degradation via a clathrin/LDLRAP1-mediated pathway, preventing recycling or directing to lysosomes, and can induce LDLR ubiquitination. Related references: PMID:17461796, PMID:18197702, PMID:18799458
• Inhibits intracellular degradation of APOB and disposes of non-acetylated BACE1 intermediates in the early secretory pathway. Related references: PMID:18660751
• Regulates epithelial Na+ channel (ENaC)-mediated Na+ absorption by increasing proteasomal degradation of ENaC.
• Modulates neuronal apoptosis via LRP8/APOER2 levels and related signaling pathways.
• Subcellular localization includes cytoplasm, secretion, endosomes, lysosomes, cell surface, endoplasmic reticulum, and Golgi apparatus.
• Expressed in neuro-epithelioma, colon carcinoma, hepatic and pancreatic cell lines, and Schwann cells.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a peptide within amino acids 590-692 of human PCSK9, which includes part of the C-terminal domain. Consider that this region is present in the full-length proprotein and may be retained in some cleaved forms.
• For Western blotting (WB), the full-length PCSK9 has a predicted molecular weight of ~74 kDa. Additional bands corresponding to processed forms (e.g., mature ~62 kDa chain and prodomain) may be observed depending on sample preparation and cellular context.
• For immunoprecipitation (IP), validate the antibody’s performance with your specific sample type, as PCSK9 can be secreted or retained intracellularly.
• For immunofluorescence (IF/ICC) and immunohistochemistry (IHC-P), fixed and permeabilized samples are recommended due to the protein’s localization in multiple organelles. Validate in positive control tissues (e.g., liver, colon carcinoma) and include appropriate controls.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a dedicated one-stop sourcing bridge for IVD raw materials, supporting researchers and diagnostic manufacturers in cholesterol metabolism and cardiovascular disease studies. In addition to high-quality PCSK9 antibodies, we offer validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents to streamline assay development. Our team also assists in sourcing rare-target raw materials, ensuring continuity from concept to clinic.