Anti-Proprotein Convertase 9 (PCSK9) Rabbit mAb for WB, ELISA - Q8NBP7

Core Product Specifications and Parameters

Biological Background: Proprotein Convertase 9 (PCSK9) Function and Localization

• PCSK9 (Proprotein Convertase Subtilisin/Kexin type 9) is a serine protease synthesized as a zymogen that undergoes autocatalytic cleavage in the endoplasmic reticulum to generate the mature enzyme.
• It critically regulates plasma cholesterol homeostasis by binding to LDL receptor family members (LDLR, VLDLR, LRP1, LRP8) and promoting their degradation in intracellular acidic compartments, primarily via a clathrin-mediated pathway. PCSK9 can also induce ubiquitination of LDLR, directing it to lysosomal degradation. Related references: PMID:18039658 PMID:17461796 PMID:18197702 PMID:18799458
• PCSK9 mediates the disposal of non-acetylated BACE1 intermediates in the early secretory pathway, impacting Alzheimer's disease-related processing. Related references: PMID:18660751
• It localizes to multiple cellular compartments including the cytoplasm, endoplasmic reticulum, Golgi, endosomes, lysosomes, cell surface, and is also secreted into the extracellular milieu.
• Tissue expression includes neuro-epithelioma, colon carcinoma, hepatic and pancreatic cell lines, and Schwann cells, with high hepatic expression being critical for its role in cholesterol metabolism.
• Post-translational modifications such as glycosylation, sulfation, phosphorylation, and disulfide bonds are essential for PCSK9 folding, secretion, and function.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide spanning amino acids 500–600 of human PCSK9. Validate specificity using appropriate negative and positive controls.
• For Western blotting, PCSK9 has a predicted molecular weight of ~74 kDa, but may appear as multiple bands due to prodomain cleavage and glycosylation. Verify in relevant tissue or cell lysates.
• For ELISA applications, consider using a matched antibody pair to ensure sensitive and specific detection of PCSK9.
• Cross-reactivity with mouse and rat has been reported; however, it is recommended to verify reactivity in your specific sample matrix.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your trusted one-stop partner for IVD raw materials, offering antibodies, antigens, and ancillary reagents for diagnostic development. This anti-PCSK9 antibody supports research into cholesterol regulation, cardiovascular disease, and lipid metabolism. Contact us for bulk purchasing or custom sourcing of rare targets and ancillary reagents.