Anti-Prolactin-inducible protein (PIP) Polyclonal Antibody for IF/ICC, ELISA - P12273

Core Product Specifications and Parameters

Biological Background: PIP Function and Localization

• Prolactin-inducible protein (PIP) is a secreted glycoprotein, also known as gross cystic disease fluid protein 15 (GCDFP-15), secretory actin-binding protein (SABP), and gp17.
• The mature protein is a single-chain polypeptide of approximately 16.5–17 kDa, generated after cleavage of the signal peptide, and contains a pyrrolidone carboxylic acid at the N-terminus along with one intramolecular disulfide bond.
• PIP possesses an actin-binding domain and is reported to interact with actin, suggesting a role in modulating the cytoskeleton or secretory pathways.
• Expression is regulated by prolactin and androgens; it is found in pathological conditions of the mammary gland, including breast cysts and tumors, and is frequently used as an immunomarker for breast cancer.
• In addition to the mammary gland, PIP is expressed in several normal exocrine tissues, such as the lacrimal, salivary, and sweat glands.
• Functional studies and protein keywords indicate involvement in secretory processes and potential immune defense, though precise biological functions remain under investigation.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the mature region (aa 29–146) of human PIP, which excludes the signal peptide; the antibody is expected to recognize the secreted form of the protein.
• For immunofluorescence/immunocytochemistry (IF/ICC), consider using permeabilization if probing for intracellular PIP, but note that the protein is primarily secreted; surface staining on live cells may be possible without permeabilization.
• In ELISA applications, this polyclonal antibody can be utilized as either capture or detection reagent; optimize coating and detection conditions for the specific assay format.
• Validate cross-reactivity carefully in your sample system; this antibody is reported to react with human PIP. Cross-reactivity with other species has not been determined.
• The observed molecular weight in Western blot may appear slightly larger than the predicted 16.5 kDa due to glycosylation.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as your comprehensive sourcing partner for IVD raw materials, offering validated antibody pairs, monoclonal and polyclonal antibodies, bulk ancillary reagents, and rare-target sourcing capabilities. This anti-PIP polyclonal antibody supports research into breast cancer biomarkers and exocrine gland physiology, and can be complemented by our portfolio of secondary antibodies, blocking buffers, and assay development consulting. Let CamelBio streamline your reagent supply chain from concept to clinic.