Anti-Prokineticin-2 Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - Q9HC23

Core Product Specifications and Parameters

Biological Background: Prokineticin-2 Function and Localization

• Prokineticin-2 (PK2) is a secreted neuropeptide encoded by the PROK2 gene, also known as Bv8 homolog.
• It functions as a critical output molecule from the suprachiasmatic nucleus (SCN) that transmits behavioral circadian rhythm.
• Endogenous PK2 may also act locally within the SCN to synchronize neuronal output.
• PK2 potently contracts gastrointestinal (GI) smooth muscle, indicating a role in gut motility.
• The protein is secreted and contains a signal peptide and multiple disulfide bonds.
• Tissue expression is highest in the testis, with low levels detected in the small intestine.
• Alternative splicing produces isoforms, and mutations are associated with Kallmann syndrome and hypogonadotropic hypogonadism.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 20–129 of mature PROK2, so the antibody may recognize all isoforms. Validate in the relevant sample system.
• For Western blotting, expect a band around 14 kDa. Use appropriate loading controls and validate with knockout or knockdown models if possible.
• For immunohistochemistry and immunofluorescence, permeabilization may be required to detect intracellular pools of this secreted protein.
• The antibody’s cross-reactivity to mouse and rat makes it suitable for rodent circadian rhythm and gastrointestinal studies.
• Consider ELISA for quantitative detection in biological fluids; optimize coating and detection conditions.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a comprehensive range of IVD raw materials for circadian rhythm and neuropeptide research, including validated polyclonal antibodies like this anti-PROK2. Beyond standard catalog products, we provide custom antibody development, bulk reagent supply, and rare-target sourcing to streamline your diagnostic and research workflows.