Anti-Prohibitin Rabbit Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - P35232

Core Product Specifications and Parameters

Biological Background: Prohibitin Function and Localization

• Prohibitin 1 (PHB; PHB1) is a highly conserved protein with pleiotropic functions, including mitochondrial chaperone activity, nuclear transcription coregulation, and plasma membrane signaling, acting in a cell-compartment- and tissue-specific manner (PubMed:11302691, PubMed:20959514, PubMed:28017329). Related references: PMID:11302691, PMID:20959514, PMID:28017329
• In mitochondria, PHB forms large ring complexes with PHB2 in the inner membrane, functioning as a chaperone that stabilizes respiratory enzymes, regulates cardiolipin remodeling, and is essential for mitochondrial morphogenesis and longevity (PubMed:11302691). This complex also serves as a mitophagy receptor (PubMed:28017329). Related references: PMID:11302691, PMID:28017329
• PHB mediates mitochondrial antiviral innate immunity by activating RIG-I signal transduction, leading to the production of type I interferon (IFNB1) and pro-inflammatory cytokine IL6 (PubMed:31522117). Related references: PMID:31522117
• In the nucleus, PHB acts as a transcriptional coregulator, enhancing TP53 promoter binding while repressing E2F1, thus influencing cell cycle and apoptosis (PubMed:14500729). It also interacts with STAT3 to regulate IL17 secretion in Th17 cells (PubMed:31899195). Related references: PMID:14500729, PMID:31899195
• At the plasma membrane, PHB cooperates with CD86 in B lymphocytes to modulate IgG1 production and is required for CD40-mediated NF-kappa-B activation.
• Widely expressed in tissues, PHB localizes to the mitochondrial inner membrane, nucleus, cytoplasm, and cell membrane. It is subject to acetylation and phosphorylation, and is implicated in host-virus interactions and cellular aging.

Experimental Guidance and Technical Tips

• The immunogen (aa 73–272) targets the central/C-terminal region of human PHB; verify sequence homology if working with other species.
• For Western blot, expect a band at ~30 kDa under standard reducing conditions; validate with positive controls (e.g., HeLa, HepG2) and consider loading controls.
• For IHC-P, antigen retrieval (e.g., citrate buffer, pH 6.0) may be necessary; optimize antibody dilution and detection system for your tissue type.
• For IF/ICC, optimize fixation/permeabilization to preserve mitochondrial and nuclear epitopes; co-staining with organelle markers is recommended.
• As a polyclonal antibody, evaluate lot-to-lot consistency for quantitative assays; consider pilot experiments for reproducibility.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research labs with comprehensive IVD raw-material supply, from validated monoclonal/polyclonal antibodies to bulk ancillary reagents. This Prohibitin antibody facilitates mitochondrial, nuclear, and immunological research, and CamelBio offers tailored sourcing solutions for target-specific proteins and rare reagents. Contact us to streamline your procurement and accelerate assay development.