Anti-Prohibitin Rabbit Polyclonal Antibody for WB, IHC-P, ELISA - P35232

Core Product Specifications and Parameters

Biological Background: Prohibitin (PHB) Function and Localization

• Prohibitin 1 (PHB) is a pleiotropic protein with cell-compartment- and tissue-specific functions, including plasma membrane-associated signaling, mitochondrial chaperone activity, and nuclear transcriptional co-regulation. Related references: PMID:11302691 PMID:20959514 PMID:28017329
• In mitochondria, PHB assembles with PHB2 into large ring complexes at the inner membrane, acting as a chaperone that stabilizes respiratory enzymes, maintains mitochondrial integrity, regulates mitophagy, and participates in antiviral innate immunity. Related references: PMID:11302691 PMID:28017329 PMID:31522117
• Nuclear PHB functions as a transcription coregulator, enhancing TP53 promoter binding while repressing E2F1, and interacts with STAT3 to influence IL-17 secretion in Th17 cells. Related references: PMID:14500729 PMID:31899195
• At the plasma membrane, PHB cooperates with CD86 in B lymphocytes to modulate IgG1 production and is required for CD40-mediated NF-kappa-B signaling activation.
• PHB localizes to the mitochondrial inner membrane, nucleus, cytoplasm, and cell membrane.
• It is widely expressed across diverse tissues.
• Key post-translational modifications and functional associations include acetylation, phosphorylation, alternative splicing, and involvement in host-virus interactions.

Experimental Guidance and Technical Tips

• The immunogen spans amino acids 73–272, a region likely accessible in native and denatured conditions, supporting detection across applications.
• For WB, expect a band near 30 kDa; additional bands or slight shifts may appear due to post-translational modifications or tissue-specific isoforms—validate with appropriate controls.
• In IHC-P, pre-test on tissues with known Prohibitin expression; widespread distribution makes both positive and negative tissue controls feasible.
• Given the polyclonal nature, consider verifying specificity using knockdown or knockout models if cross-reactivity is suspected.
• Subcellular fractionation or organelle enrichment can improve detection in compartment-specific studies, especially for mitochondrial or nuclear pools.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research labs with end-to-end access to IVD raw materials, covering every stage from concept to clinic. For Prohibitin-related projects in mitochondrial biology and cell signaling, we supply validated antibody pairs, optimized polyclonal and monoclonal antibodies, bulk ancillary reagents, and can assist with rare-target raw-material sourcing—streamlining your procurement and accelerating assay development. Let CamelBio be your trusted partner in securing high-quality, research-grade immunological tools.