Anti-Prohibitin Rabbit Monoclonal Antibody for WB - P35232

Core Product Specifications and Parameters

Biological Background: Prohibitin Function and Localization

• Prohibitin (PHB1, PHB) is a pleiotropic protein with functions in mitochondria, nucleus, and plasma membrane, playing roles in cell signaling, mitochondrial integrity, and transcriptional regulation.
• In mitochondria, PHB forms large ring complexes with PHB2 in the inner mitochondrial membrane, acting as a chaperone that stabilizes respiratory enzymes and maintains mitochondrial morphogenesis, neuronal survival, and normal lifespan. The prohibitin complex regulates cardiolipin remodeling and OMA1 turnover, and functions as a mitophagy receptor targeting mitochondria for autophagic degradation.
  Related references: PMID:11302691 PMID:28017329
• PHB is involved in mitochondrial-mediated antiviral innate immunity, activating RIG-I signal transduction and production of IFNB1 and IL6.
  Related references: PMID:31522117
• In the nucleus, PHB acts as a transcription coregulator: it enhances TP53 promoter binding (activating it) and reduces E2F1 promoter binding (repressing it). It also interacts with STAT3 to affect IL17 secretion in Th17 cells.
  Related references: PMID:14500729 PMID:31899195
• At the plasma membrane, PHB cooperates with CD86 to mediate CD86-signaling in B lymphocytes, regulating IgG1 production, and upon CD40 engagement, activates NF-kappa-B signaling via PLC and PKC.
• PHB contributes to adipose tissue and glucose homeostasis in a sex-specific manner, and to pulmonary vascular remodeling by accelerating proliferation of pulmonary arterial smooth muscle cells.
• Subcellularly, PHB localizes to the mitochondrion inner membrane, nucleus, cytoplasm, and cell membrane. It is widely expressed in different tissues.
• Key post-translational modifications and features include acetylation, phosphorylation, and alternative splicing; it is classified as a proto-oncogene and is involved in host-virus interactions, DNA synthesis, and mitochondrial organization.

Experimental Guidance and Technical Tips

• This antibody is generated by immunizing rabbits with a synthetic peptide corresponding to amino acids 100-200 of human Prohibitin (P35232). For Western blot (WB), a band around 30kDa is expected; verify specificity using appropriate positive and negative control lysates.
• For immunohistochemistry (IHC-P), optimize antigen retrieval and antibody dilution using your tissue of interest; consider using a known positive control tissue such as liver or kidney, where Prohibitin is widely expressed.
• In ELISA applications, determine the optimal coating concentration and detection range experimentally; the monoclonal nature ensures high batch-to-batch consistency.
• As the antibody cross-reacts with mouse and rat, it can be used in rodent models; validate in the relevant sample system to ensure performance.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers, labs, and research institutes a streamlined pathway to IVD raw materials, from concept to clinic. This anti-Prohibitin monoclonal antibody is part of CamelBio's extensive portfolio of validated antibodies targeting key proteins in mitochondrial biology, cell signaling, and oncology research. Beyond antibodies, CamelBio provides validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and custom sourcing of hard-to-find raw materials—all supported by technical and regulatory expertise to accelerate your diagnostic development.