Anti-Prohibitin 2 (PHB2) Rabbit Polyclonal Antibody for WB, IHC-P, IP, ELISA - Q99623

Core Product Specifications and Parameters

Biological Background: Prohibitin 2 Function and Localization

• Prohibitin-2 (PHB2), also known as BAP37, REA, or repressor of estrogen receptor activity, is a pleiotropic protein with cell-compartment- and tissue-specific functions.
• In mitochondria, PHB2 forms large ring complexes with PHB1 in the inner membrane, stabilizing respiratory enzymes and maintaining mitochondrial integrity. It regulates cytochrome-c oxidase assembly, acts as a mitophagy receptor, and mediates antiviral innate immunity by activating RIG‑I signaling. Related references: PMID:11302691 PMID:20959514 PMID:28017329 PMID:31522117
• In the nucleus, PHB2 functions as a transcriptional co-regulator, mediating repression by nuclear hormone receptors through histone deacetylase recruitment. It acts as an estrogen receptor-selective coregulator, potentiating antiestrogen effects and repressing estrogen activity.
• At the plasma membrane, PHB2 is involved in IGFBP6-induced cell migration and cooperates with CD86 in B lymphocytes to regulate IgG1 production. Upon CD40 engagement, it activates NF-kappa‑B signaling. Related references: PMID:24003225
• PHB2 is involved in human enterovirus 71 (EV‑71) infection by enhancing autophagy during the viral life cycle.
• Subcellularly, PHB2 localizes to the mitochondrial inner membrane, cytoplasm, nucleus, and cell membrane. It is expressed in myoblasts and undergoes phosphorylation and acetylation.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 1‑299 of human PHB2 (NP_001138303.1), covering the N‑terminal region. Verify sequence conservation with mouse and rat orthologs to support cross‑reactivity claims.
• For WB, the predicted molecular weight is ~33 kDa. Use appropriate positive controls, such as human cell lysates, and validate antibody specificity.
• For IHC‑P, consider optimizing antigen retrieval and antibody dilution using tissue known to express PHB2 (e.g., skeletal muscle).
• For IP, optimize antibody concentration and incubation conditions to ensure efficient capture of endogenous PHB2.
• For ELISA, recombinant protein or peptide corresponding to the immunogen can be used as coating antigen.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting. For targets like Prohibitin 2, which plays critical roles in mitochondrial biology, transcriptional regulation, and cancer, CamelBio offers validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and rare‑target raw‑material sourcing to support your entire workflow from concept to clinic.