Anti-Progranulin (Granulin) Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - P28799

Core Product Specifications and Parameters

Biological Background: Progranulin Function and Localization

• Progranulin (Granulin, GP88, PCDGF) is a secreted glycoprotein encoded by the GRN gene that acts as a key regulator of lysosomal function and as a growth factor involved in inflammation, wound healing, and cell proliferation. Related references: PMID:12526812 PMID:18378771 PMID:28073925
• It regulates protein trafficking to lysosomes and the activity of lysosomal enzymes, thereby facilitating the degradation of mature CTSD by CTSB. Related references: PMID:28453791 PMID:28541286
• As a wound-related growth factor, Progranulin directly promotes division, migration, and capillary-like tubule formation in dermal fibroblasts and endothelial cells.
• The protein blocks TNF-mediated neutrophil activation, preventing the release of oxidants and proteases that would otherwise damage epithelial cells. Related reference: PMID:12526812
• In the nervous system, Progranulin preserves neuronal survival, axonal outgrowth, and integrity, thereby modulating neuroinflammation. Related reference: PMID:18378771
• Subcellularly, Progranulin is found both secreted and within lysosomes, consistent with its dual role in extracellular signaling and intracellular degradation.
• Tissue expression is highest in kidney, with prominent presence in fibroblasts, epithelial cell lines, inflammatory cells, and bone marrow.

Experimental Guidance and Technical Tips

• The immunogen spans amino acids 20–310 of human Progranulin, covering the full-length precursor; it may recognize both the unprocessed protein and potentially processed granulin peptides. Consider validating selectivity in the relevant sample system.
• For western blotting, the predicted molecular weight of full-length Progranulin is ~64 kDa. Processed granulin peptides may appear as smaller bands; use appropriate positive and negative controls.
• In immunofluorescence/immunocytochemistry, note that Progranulin is primarily secreted and localized to lysosomes. Permeabilization and co-staining with lysosomal markers may enhance detection.
• Given the confirmed cross-reactivity with mouse, this antibody can be useful for studies in mouse models of inflammation, wound healing, or lysosomal storage disorders. Always validate in the intended species and application.

CamelBio: Your One-Stop Sourcing Bridge

This anti-Progranulin antibody supports research into wound healing, neuroinflammation, and lysosomal dysfunction. CamelBio partners with diagnostic manufacturers and laboratories to provide one-stop access to IVD raw materials—including validated antibody pairs, bulk ancillary reagents, and customized sourcing for rare targets. Whether scaling up your assay or tackling challenging biomarkers, we bridge the gap from concept to clinic.