Anti-Progesterone Receptor Rabbit Monoclonal Antibody for WB, ELISA - P06401

Core Product Specifications and Parameters

Biological Background: Progesterone Receptor Function and Localization

• Progesterone receptor (PR), encoded by the PGR gene, is a member of the nuclear receptor subfamily 3 group C (NR3C3). Two major isoforms, A and B, are produced via alternative promoter usage and splicing, differing in their N-terminal regions.
• PR functions as a ligand-activated transcription factor that regulates gene expression in response to progesterone, controlling cellular proliferation and differentiation in target tissues.
• Depending on the isoform, PR can act as a transcriptional activator or repressor. Isoform B typically mediates transcriptional activation, while isoform A can function as a transdominant repressor of other steroid hormone receptors, including estrogen receptor and mineralocorticoid receptor.
• Upon hormone binding, PR activates SRC-dependent MAPK signaling, contributing to rapid non-genomic effects.
• PR has been shown to localize to the nucleus, cytoplasm, and mitochondrial outer membrane. Its subcellular distribution may vary with isoform and cellular context.
• In reproductive tissues, isoform A and B show differential expression patterns throughout the menstrual cycle, with isoform A predominating in the stroma and isoform B persisting in glandular epithelium during the mid-secretory phase.
• Post-translational modifications include phosphorylation, palmitoylation, and SUMOylation, which modulate receptor stability, localization, and transcriptional activity.
• Progesterone signaling through PR is implicated in increasing mitochondrial membrane potential and cellular respiration, highlighting a role in metabolic regulation.

Experimental Guidance and Technical Tips

• The immunogen encompasses amino acids 600–700, a region conserved in both PR-A and PR-B isoforms; therefore, the antibody is predicted to recognize both isoforms.
• For Western blotting, the predominant band for PR-B migrates near 99 kDa, while PR-A is typically observed at approximately 94 kDa. Validate specificity using positive and negative cell lines.
• Optimal antibody dilutions and incubation conditions should be empirically determined for each experimental system.
• For ELISA applications, consider coating recombinant PR protein or nuclear extracts from progesterone-responsive cells to assess antibody performance.
• Always include appropriate controls and confirm target identity via secondary methods when exploring novel sample types.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a one-stop sourcing partner for IVD raw materials, offering comprehensive support from early research to clinical application. Our portfolio includes high-performance monoclonal antibodies like this progesterone receptor antibody, ideal for reproductive biology and hormone receptor research. Beyond antibodies, CamelBio can supply validated antibody pairs, optimized reagents, and assist with sourcing rare targets to streamline your assay development.