Anti-Profilin1 Rabbit Monoclonal Antibody for WB, ELISA - P07737

Core Product Specifications and Parameters

Biological Background: Profilin-1 Function and Localization

• Profilin-1, also known as profilin I or epididymis tissue protein Li 184a, is a small actin-binding protein encoded by the PFN1 gene.
• At high concentrations, profilin-1 prevents actin polymerization, while at low concentrations it enhances polymerization, thereby regulating cytoskeleton dynamics.
• It binds to phosphatidylinositol 4,5-bisphosphate (PIP2) and inhibits the formation of inositol trisphosphate (IP3) and diacylglycerol (DG).
• Profilin-1 inhibits androgen receptor (AR) and huntingtin (HTT) aggregation, with G-actin binding essential for AR inhibition.
• The protein is localized in the cytoplasm and associated with the cytoskeleton.
• It is expressed in epididymis, among other tissues.
• Keywords highlight its involvement in amyotrophic lateral sclerosis and neurodegeneration, and it undergoes modifications such as acetylation, phosphorylation, and ubiquitination.

Experimental Guidance and Technical Tips

• For Western blotting, the expected molecular weight of Profilin-1 is approximately 15 kDa. Use appropriate gel percentage and transfer conditions optimized for small proteins.
• Include a positive control lysate from human, mouse, or rat cell lines known to express PFN1 to confirm reactivity.
• For ELISA, validate the antibody pairing with a suitable detection antibody and optimize coating concentrations.
• Optimize blocking and washing steps to minimize non-specific binding.
• The immunogen sequence (amino acids 61–140 of human PFN1) is conserved across human, mouse, and rat, supporting the reported cross-reactivity; however, experimental validation in your specific sample system is recommended.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers, laboratories, and research institutes a streamlined, end-to-end sourcing solution for IVD raw materials. For Profilin-1 and related cytoskeletal targets, we supply validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and can assist in sourcing rare target raw materials. By partnering with CamelBio, you gain a reliable bridge from concept to clinic, backed by technical consultation and comprehensive supply chain support.