Anti-Profilin1 Polyclonal Antibody for WB and ELISA - P07737

Core Product Specifications and Parameters

Biological Background: Profilin-1 Function and Localization

• Profilin-1 is a small actin-binding protein that regulates cytoskeletal dynamics. At high concentrations, it prevents actin polymerization, while at low concentrations, it promotes polymerization.
• It binds to phosphatidylinositol 4,5-bisphosphate (PIP2), inhibiting the formation of inositol triphosphate (IP3) and diacylglycerol (DG), thereby modulating signal transduction pathways.
• Profilin-1 inhibits androgen receptor (AR) and huntingtin (HTT) aggregation; its G-actin binding activity is essential for AR inhibition.
• The protein localizes to the cytoplasm and cytoskeleton.
• It is expressed in the epididymis at the protein level.
• Post-translational modifications include acetylation, phosphorylation, and ubiquitination.
• Genetic variants in PFN1 are associated with amyotrophic lateral sclerosis (ALS18), linking profilin-1 to neurodegeneration.

Experimental Guidance and Technical Tips

• The immunogen covers the full-length human Profilin-1 protein (aa 1-140). For western blot, expect a band at approximately 15 kDa; use low-molecular-weight markers and validate detection in your sample system.
• The antibody cross-reacts with human, mouse, rat, and monkey, allowing flexibility in model organism selection. Consider preliminary testing to confirm reactivity in your specific tissue or cell line.
• For ELISA applications, optimize coating and detection conditions using a known positive control (e.g., recombinant Profilin-1) to establish assay sensitivity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research labs with one-stop access to validated IVD raw materials, including high-quality antibodies for cytoskeletal targets like profilin-1. Our portfolio covers polyclonal antibodies, recombinant proteins, and ancillary reagents, supported by technical consultation from concept to clinic. For bulk orders, customized antibody development, or sourcing rare targets, contact our team to streamline your workflow.