Anti-Proenkephalin-A Monoclonal Antibody for WB, IF-P, ELISA - P01210

Core Product Specifications and Parameters

Biological Background: Proenkephalin-A Function and Localization

• Proenkephalin-A (PENK) is a precursor protein that is proteolytically processed to generate several active neuropeptides, including Met-enkephalin and Leu-enkephalin, which belong to the endogenous opioid peptide family.
• The derived opioid peptides compete with and mimic the effects of opiate drugs, playing critical roles in physiologic processes such as pain perception and responses to stress.
• Met-enkephalin-Arg-Phe, one of the processed peptides, acts as a strong ligand of the Mu-type opioid receptor OPRM1, modulating glutamatergic and GABAergic neurotransmission in the striatum.
• Proenkephalin-A-derived peptides also exhibit non-opioid actions: they serve as ligands for the MRGPRX1 receptor, inducing histamine-independent itch and nociceptive sensations in sensory neurons. Related references: PMID:11850634, PMID:21593341, PMID:28223516
• Subcellularly, proenkephalin-A is localized to the lumen of secretory vesicles, particularly chromaffin granules, and is secreted upon stimulation.
• The protein undergoes post-translational modifications including disulfide bond formation and phosphorylation, and is characterized by cleavage at pairs of basic residues to release mature peptides.
• Keywords associated with PENK include neuropeptide, opioid peptide, endorphin, and secreted, consistent with its role in cell-to-cell signaling and pain modulation.

Experimental Guidance and Technical Tips

• The immunogen was designed using a recombinant fragment covering amino acids 25-267 of human PENK, which corresponds to the proenkephalin-A precursor region. This design may help generate antibodies recognizing multiple processed forms.
• For WB, a band at ~31 kDa is expected; however, due to proteolytic processing, additional lower molecular weight bands may be observed. Consider optimizing sample preparation to preserve neuropeptide integrity.
• For IF-P, validate fixation and permeabilization conditions in your cell or tissue system, as the protein is secreted and stored in vesicles.
• For ELISA, pairing with other clones or antibodies may be necessary to establish a sandwich assay.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is dedicated to supporting diagnostic manufacturers and research institutions with comprehensive IVD raw-material solutions. This Proenkephalin-A antibody is part of our expanding portfolio for neuroscience and pain research, complemented by validated antibody pairs, optimized monoclonal antibodies, and bulk ancillary reagents. For rare or specialized targets like opioid peptides, CamelBio offers reliable sourcing and technical consultation to streamline your assay development from concept to clinic.