Anti-PRODH Rabbit Monoclonal Antibody for WB, IP, IHC-P, ELISA - O43272

Core Product Specifications and Parameters

Biological Background: PRODH Function and Localization

• PRODH (proline dehydrogenase 1), also known as proline oxidase, POX, PIG6, or HSPOX2, is a mitochondrial enzyme encoded by the PRODH gene in humans.
• It catalyzes the first step in proline degradation, converting L-proline to delta-1-pyrroline-5-carboxylate (P5C), a critical reaction linking proline metabolism to the urea cycle and the TCA cycle.
• As an FAD-dependent oxidoreductase, PRODH transfers electrons from proline directly to the mitochondrial electron transport chain, contributing to ATP production and reactive oxygen species generation.
• The protein is localized to the mitochondrial matrix, consistent with its role in amino acid catabolism and energy metabolism.
• PRODH is expressed predominantly in lung, skeletal muscle, and brain, with lower levels in heart and kidney, and weak expression in liver, placenta, and pancreas.
• Post-translational modifications include acetylation, potentially regulating enzyme activity or stability; genetic variations and deficiency are associated with hyperprolinemia type I and schizophrenia susceptibility.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 321–600 of human PRODH, a region that is well conserved across species; this supports reactivity with mouse and rat orthologs.
• For western blotting, consider starting with a 1:500–1:2000 dilution and adjust based on target abundance in your sample; the expected band size is approximately 68 kDa.
• In immunoprecipitation, optimize antibody–bead conjugation and lysis buffer conditions to preserve native protein interactions; include appropriate IgG controls.
• For immunohistochemistry (paraffin-embedded sections), antigen retrieval with citrate buffer (pH 6.0) is often beneficial; validate staining specificity with known positive and negative tissues.
• ELISA performance may require pairing with a suitable detection antibody; test linearity and sensitivity using recombinant PRODH protein as standard.

CamelBio: Your One-Stop Sourcing Bridge

As a one-stop IVD raw-material partner, CamelBio connects diagnostic manufacturers and research teams with validated antibodies, antigen pairs, and ancillary reagents for seamless assay development. This anti-PRODH monoclonal antibody supports investigations into proline metabolism, mitochondrial dysfunction, and disease biomarkers, and can be sourced alongside optimized bulk reagents and rare-target antibodies through CamelBio’s comprehensive supply network.