Anti-PRMT9 Rabbit Polyclonal Antibody for WB, ELISA - Q6P2P2

Core Product Specifications and Parameters

Biological Background: PRMT9 Function and Localization

• PRMT9 is an arginine methyltransferase that catalyzes both monomethylation and symmetrical dimethylation, specifically targeting SF3B2 to regulate alternative splicing of pre-mRNA.
  Related references: PMID:25737013, PMID:25979344
• It is predominantly localized in the cytoplasm.
• The protein features tetratricopeptide (TPR) repeats and a methyltransferase domain, indicating its role as a modifying enzyme.
• The calculated molecular weight is 94.5 kDa, with the mature protein observed at approximately 95 kDa.
• PRMT9 is also known as PRMT10 and is involved in post-translational protein modification and RNA processing.

Experimental Guidance and Technical Tips

• This antibody was raised against an N-terminal fragment (aa 1-170) of human PRMT9; consider this when analyzing truncated or isoform-specific signals.
• For Western blotting, a band of approximately 95 kDa is expected in human cell lysates; validate with appropriate positive and negative controls.
• Optimize antibody dilution for ELISA by performing a titration series; recommended starting dilutions might range from 1:500 to 1:2000 for WB and 1:1000 to 1:5000 for ELISA.
• Given the polyclonal nature, lot-to-lot consistency should be confirmed for quantitative assays; always run a loading control.
• The antibody is reactive with human PRMT9; cross-reactivity with other species has not been tested.

CamelBio: Your One-Stop Sourcing Bridge

For researchers investigating protein arginine methylation and alternative splicing, CamelBio offers one-stop access to high-quality IVD raw materials like the PRMT9 rabbit polyclonal antibody, alongside a broad range of ancillary reagents, validated antibody pairs, and custom sourcing of rare targets. With a commitment to supporting the entire development pipeline from concept to clinic, we provide tailored consulting and supply services to meet your unique project requirements.