Anti-PRMT8 Rabbit Polyclonal Antibody for WB and ELISA - Q9NR22

Core Product Specifications and Parameters

Biological Background: PRMT8 Function and Localization

• PRMT8 (Protein arginine N-methyltransferase 8) is a 45 kDa membrane-associated enzyme belonging to the protein arginine methyltransferase family, also designated HRMT1L3, HRMT1L4, and PRMT8.
• It is a brain-specific methyltransferase, with expression primarily restricted to the central nervous system.
• PRMT8 catalyzes the formation of monomethylarginine (MMA) and asymmetric dimethylarginine (aDMA) on substrates including histone H4, H2A, and H2A/H2B dimers, playing a role in chromatin remodeling and transcriptional regulation. Related references: PMID:16051612 PMID:17925405 PMID:26529540
• It also methylates EWS protein, although the biological outcome of this interaction is not fully understood. Related references: PMID:18320585
• The protein is anchored to the cell membrane via an N-terminal myristoylation motif, which is critical for its localization and function.
• Alternative splicing produces multiple isoforms, which may modulate its activity or substrate specificity.
• Structural studies have revealed a conserved methyltransferase domain and a unique N-terminal extension that distinguishes it from other family members.

Experimental Guidance and Technical Tips

• Given the immunogen encompasses amino acids 200-380 within the catalytic domain, the antibody is expected to recognize full-length PRMT8 and potentially some splice isoforms. Validate in your specific model system.
• For Western blot, consider using brain tissue lysates (human, mouse, or rat) as positive controls, since PRMT8 expression is brain-specific.
• In ELISA applications, as this is a polyclonal antibody, pairing with a PRMT8-specific monoclonal antibody may improve assay specificity if developing a sandwich ELISA.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio partners with diagnostic manufacturers, labs, and research institutes to streamline IVD raw-material procurement from concept to clinic. Our portfolio includes validated antibody pairs, optimized polyclonal antibodies, and bulk ancillary reagents for epigenetic and neurological research targets like PRMT8. We also specialize in rare target sourcing and technical consultation to accelerate your assay development.