Anti-PRMT7 Rabbit Monoclonal Antibody for WB, ELISA - Q9NVM4

Core Product Specifications and Parameters

Biological Background: PRMT7 Function and Localization

• Protein arginine N-methyltransferase 7 (PRMT7) belongs to the family of protein arginine methyltransferases, which catalyze the transfer of methyl groups from S-adenosyl-L-methionine to arginine residues. It can generate both omega-N monomethylarginine (MMA) and symmetrical dimethylarginine (sDMA), with a preference for MMA formation.
• PRMT7 specifically methylates arginine residues in small nuclear ribonucleoproteins Sm D1 (SNRPD1) and Sm D3 (SNRPD3), a modification required for the assembly and biogenesis of snRNP core particles, essential for pre-mRNA splicing.
• It mediates symmetric dimethylation of histone H4 at Arg-3 (H4R3me2s), a mark involved in transcriptional regulation. This activity is recruited by CTCFL at the H19 imprinted control region (ICR), contributing to gene imprinting by potentially recruiting DNA methyltransferases.
• PRMT7 may play a role in embryonic stem cell (ESC) pluripotency, though the exact mechanisms remain to be fully elucidated.
• In vitro, PRMT7 can methylate histone H2A and myelin basic protein (MBP), but the in vivo relevance of these activities is unclear.
• The protein localizes to both the cytoplasm and the nucleus (cytosol and nuclear compartments), consistent with its roles in cytoplasmic snRNP assembly and nuclear chromatin regulation.
• Alternative splicing generates multiple isoforms, which may have distinct functions or expression patterns. The gene is associated with disease variants including dwarfism and intellectual disability, underscoring its biological importance.
• Keywords: Alternative splicing, Chromatin regulator, Cytoplasm, Differentiation, Disease variant, Dwarfism, Intellectual disability, Methyltransferase, Nucleus, Transcription regulation, Transferase.

Experimental Guidance and Technical Tips

• The immunogen used was a synthetic peptide corresponding to amino acids 1-100 of human PRMT7. When performing Western blot, consider that the predicted molecular weight is 78 kDa, but post-translational modifications or isoform differences may cause slight shifts. Validate the antibody in the relevant sample system using appropriate positive and negative controls.
• For Western blot, standard protocols can be applied, but optimization of blocking conditions and antibody dilutions is recommended. For ELISA, pairing with a suitable detection antibody may be necessary for sandwich assays.
• Cross-reactivity is reported for human, mouse, and rat. When using non-human species, consult sequence alignment to confirm epitope conservation. Verify specificity in your experimental model.
• As PRMT7 is involved in chromatin regulation and snRNP biogenesis, cell fractionation or nuclear/cytoplasmic extraction may be useful to enrich the target and improve signal-to-noise ratio.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting, supporting projects from concept to clinic. The PRMT7 monoclonal antibody fits into epigenetics and RNA processing research areas; we supply validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and can assist with rare target raw-material sourcing. Streamline your procurement and accelerate development with CamelBio’s integrated supply solutions.