Anti-PRMT7 Polyclonal Antibody for WB, IHC-P, ELISA - Q9NVM4

Core Product Specifications and Parameters

Biological Background: PRMT7 Function and Localization

• PRMT7 (protein arginine N-methyltransferase 7) is a member of the protein arginine methyltransferase family and catalyzes the formation of omega-N monomethylarginine (MMA) and symmetrical dimethylarginine (sDMA), with a preference for MMA.
• It specifically mediates symmetric dimethylation of histone H4 at Arg-3 (H4R3me2s), a modification involved in chromatin regulation and gene imprinting.
• PRMT7 dimethylates the small nuclear ribonucleoproteins Sm D1 and Sm D3, a step required for snRNP core particle assembly and biogenesis.
• In the context of imprinting, PRMT7 is recruited by CTCFL to the H19 imprinted control region, where it methylates histone H4 and may help recruit DNA methyltransferases.
• The enzyme may also play a role in embryonic stem cell pluripotency and differentiation.
• Subcellularly, PRMT7 localizes to both the cytoplasm (cytosol) and the nucleus, consistent with its diverse substrate targets.
• In vitro, PRMT7 can methylate histone H2A and myelin basic protein, though the in vivo relevance remains unclear.
• Additional keywords associated with PRMT7 include alternative splicing, disease variants linked to dwarfism and intellectual disability, and S-adenosyl-L-methionine-dependent methylation.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 513–692 of human PRMT7; consider its C-terminal location and potential isoform specificity when designing detection or blocking strategies.
• For Western blot, the predicted molecular weight is 78kDa; verify band size under reducing conditions using appropriate protein ladders and loading controls.
• In immunohistochemistry (IHC-P), optimize antigen retrieval and antibody dilution based on tissue type and fixation conditions; including known positive and negative control tissues is advisable.
• For ELISA, use recombinant PRMT7 protein or peptide immunogen as capture antigen; validate assay conditions with a standard curve and appropriate blanks.
• As a polyclonal antibody, lot-to-lot consistency should be confirmed by testing on positive control samples; consider using affinity-purified IgG for critical applications.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers and research laboratories with reliable sourcing of IVD raw materials, including validated antibody pairs, optimized polyclonal antibodies, and bulk ancillary reagents. Our portfolio spans key epigenetic and chromatin targets, such as PRMT7, enabling developers to accelerate assay development from concept to clinic. For rare or custom targets, our technical team assists with material qualification and supply chain solutions, helping you secure the raw materials your projects require.