Anti-PRMT7 Polyclonal Antibody for WB, ELISA - Q9NVM4

Core Product Specifications and Parameters

Biological Background: PRMT7 Function and Localization

• PRMT7 (Protein arginine N-methyltransferase 7) is a member of the protein arginine methyltransferase family, also designated as SBIDDS.
• Catalyzes both monomethylarginine (MMA) and symmetric dimethylarginine (sDMA) formation, exhibiting a preference for MMA generation.
• Symmetrically dimethylates histone H4 at Arg-3 (H4R3me2s), an epigenetic mark involved in chromatin regulation and gene imprinting.
• Methylates small nuclear ribonucleoproteins Sm D1 (SNRPD1) and Sm D3 (SNRPD3); this modification is essential for snRNP core particle assembly and biogenesis.
• In vitro, methylates histone H2A and myelin basic protein (MBP), although the in vivo significance remains to be fully established.
• Recruited by CTCFL at the H19 imprinted control region (ICR), where H4R3me2s may help recruit DNA methyltransferases.
• Localizes to both cytosol and nucleus, consistent with its roles in cytoplasmic and nuclear processes.
• Annotated with keywords including alternative splicing, chromatin regulator, methyltransferase, and disease variant (associated with dwarfism and intellectual disability).

Experimental Guidance and Technical Tips

• The immunogen maps to the C-terminal region (aa 513–692) of human PRMT7; the antibody may detect full-length PRMT7 and any isoforms or fragments containing this region.
• For Western blotting, consider reducing and denaturing sample conditions; the predicted molecular weight is 78 kDa, so use a molecular weight marker spanning the 70–100 kDa range.
• Cross-reactivity with mouse and rat PRMT7 has been reported; validate specificity and sensitivity in the relevant sample system before quantitative analyses.
• This antibody has been tested for WB and ELISA; other applications (e.g., IHC, IF) may require in‑house optimization.
• In ELISA, the recombinant protein immunogen can serve as a positive control to help establish a standard curve.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers and research laboratories a streamlined one‑stop solution for IVD raw materials, including validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and sourcing for rare targets like PRMT7. With a focus on epigenetic and chromatin research, our supply chain and technical expertise support projects from early development to clinical application. Partner with CamelBio to access reliable, high‑quality raw materials and accelerate your PRMT7‑related assay development.