Anti-PRMT6 Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - Q96LA8

Core Product Specifications and Parameters

Biological Background: PRMT6 Function and Localization

• PRMT6 is a protein arginine N-methyltransferase that catalyzes both monomethylation and asymmetric dimethylation of arginine residues, with a strong preference for asymmetric dimethylation (aDMA). Related references: PMID:17898714 PMID:18077460 PMID:18079182
• It specifically methylates histone H3 at arginine 2 (H3R2me2a), an epigenetic mark linked to transcriptional repression that is mutually exclusive with H3K4 methylation. Related references: PMID:17898714 PMID:18077460 PMID:18079182
• Acts as a transcriptional repressor of genes such as HOXA2, THBS1, and TP53, playing a role in cellular senescence regulation. Related references: PMID:19509293
• Methylates non-histone substrates including DNA polymerase beta (POLB) to enhance DNA repair, HMGA1, and SIRT7, regulating diverse cellular processes. Related references: PMID:16600869 PMID:16157300 PMID:16159886 PMID:30420520
• May contribute to innate immunity by methylating HIV-1 proteins Tat, Rev, and NC, thereby impairing their function. Related references: PMID:17267505
• Localized in the nucleus and highly expressed in kidney and testis.

Experimental Guidance and Technical Tips

• The immunogen covers full-length PRMT6 (aa 1–375), so the antibody may recognize multiple isoforms or splice variants. Validate specificity in your system.
• For Western blot, expect a band around 42 kDa. Use human cell lysates as a positive control and optimize primary antibody dilution to minimize non-specific binding.
• In immunofluorescence/ICC, anticipate nuclear staining. Include proper fixation and permeabilization steps, and confirm localization with a nuclear marker.
• ELISA suitability is indicated; consider pairing with appropriate coating buffers and secondary detection reagents.
• Cross-reactivity is validated for human; if used with other species, conduct your own verification.
• As a polyclonal preparation, lot-to-lot consistency should be monitored. Include negative controls (e.g., knockout lysates) to confirm signal specificity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a dedicated one-stop sourcing bridge for IVD raw materials, connecting diagnostic manufacturers, labs, and research institutes with high-quality reagents like this PRMT6 rabbit polyclonal antibody. Our portfolio supports epigenetic and transcription regulation research, featuring validated antibodies, bulk ancillary reagents, and custom rare-target sourcing. Whether you need established polyclonal antibodies or optimized monoclonal pairs for assay development, CamelBio streamlines your procurement from concept to clinic.