Anti-PRMT6 Polyclonal Antibody for WB, IF/ICC, ELISA - Q96LA8

Core Product Specifications and Parameters

Biological Background: PRMT6 Function and Localization

• PRMT6 is a protein arginine N-methyltransferase that catalyzes asymmetric dimethylarginine (aDMA) formation, with a strong preference for aDMA over monomethylarginine (MMA). It targets arginine residues within glycine-arginine-rich motifs. Related references: PMID:17898714 PMID:18077460 PMID:18079182

• PRMT6 specifically mediates asymmetric dimethylation of histone H3 at arginine 2 (H3R2me2a), a mark linked to transcriptional repression and mutually exclusive with activating H3K4 methylation. It also methylates histones H2A and H4 at arginine 3. Related references: PMID:17898714 PMID:18077460 PMID:18079182

• PRMT6 acts as a transcriptional repressor of genes such as HOXA2, THBS1, and TP53, linking it to cellular senescence regulation. It also serves as a coactivator for steroid hormone receptors (ESR1, ESR2, PGR, NR3C1) and promotes gluconeogenic gene expression via CRTC2 methylation. Related references: PMID:19509293

• PRMT6 regulates DNA base excision repair by methylating DNA polymerase beta (POLB), enhancing its DNA binding and processivity. Related references: PMID:16600869

• It methylates non-histone substrates including HMGA1, HIV-1 proteins (Tat, Rev, NC), GPS2, and SIRT7, affecting processes such as chromatin architecture, antiviral defense, and mitochondrial biogenesis. Related references: PMID:16157300 PMID:16159886 PMID:17267505 PMID:30420520

• PRMT6 is highly expressed in kidney and testis, and localizes predominantly to the nucleus.

• PRMT6 is subject to phosphorylation and ubiquitin conjugation, and participates in alternative splicing regulation.

Experimental Guidance and Technical Tips

• The immunogen (recombinant protein covering amino acids 1-375) corresponds to the full-length PRMT6, which may generate a polyclonal response against multiple epitopes.
• In WB, a single band near 42 kDa is expected. Validate expression in cell lines or tissues known to express PRMT6, such as kidney or testis.
• For IF/ICC, nuclear localization should be observed. Include appropriate controls such as antigen-blocking peptides or PRMT6 knockout cell lines to confirm specificity.
• ELISA applications may be developed using the antibody as a capture or detection reagent; consider optimizing coating conditions and including a standard curve with recombinant PRMT6.
• Given the cross-reactivity with mouse, this antibody may be used to analyze PRMT6 in mouse samples; however, perform species-specific validation to ensure signal specificity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as your one-stop IVD raw-material sourcing partner, bridging research and diagnostic development. This anti-PRMT6 polyclonal antibody supports studies into arginine methylation, transcriptional regulation, and DNA repair. We also supply validated antibody pairs, optimized polyclonal/monoclonal antibodies, bulk ancillary reagents, and can assist in sourcing rare targets to meet your assay development and production needs.