Anti-PRMT5 Polyclonal Antibody for WB, IF/ICC, ELISA - O14744

Core Product Specifications and Parameters

Biological Background: PRMT5 Function and Localization

• PRMT5 is a type II protein arginine methyltransferase that catalyzes ω-N monomethylarginine (MMA) and symmetric dimethylarginine (sDMA) formation, with a preference for MMA (PubMed:10531356, 11152681, 11747828). Related references: PMID:10531356 PMID:11152681 PMID:11747828
• It symmetrically dimethylates Sm proteins (SNRPD1, SNRPD3) and other splicing factors (NCL, POLR2A), which is critical for snRNP assembly, spliceosome maturation, and R-loop resolution during transcription termination (PubMed:11747828, 12411503, 17709427, 21081503, 26700805). Related references: PMID:11747828 PMID:12411503 PMID:17709427 PMID:21081503 PMID:26700805
• PRMT5 methylates histone H4 at Arg-3 (and H2A/H3 at specific arginines), acting as a transcriptional corepressor at genes such as γ-globin (HBG1/HBG2) and glioblastoma-associated loci (PubMed:25092918, 25284789). Related references: PMID:25092918 PMID:25284789
• It methylates various non-histone substrates, including EGFR, RAF1, p53, HOXA9, and SRGAP2, thereby regulating EGF signaling, protein stability, cell migration, and gene transcription (PubMed:21258366, 21917714, 19011621, 22269951, 20810653). Related references: PMID:21258366 PMID:21917714 PMID:19011621 PMID:22269951 PMID:20810653
• PRMT5 localizes to the cytoplasm, nucleus, chromosome, and Golgi apparatus, and plays a role in Golgi ribbon formation through GM130 methylation (PubMed:20421892). Related references: PMID:20421892
• Ubiquitously expressed, PRMT5 is involved in cytokine signaling, cell proliferation control, and circadian clock regulation.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 538–637 of human PRMT5; the antibody recognizes an internal region and may detect full-length PRMT5 at an expected molecular weight of ~73 kDa in Western blot.
• For WB, consider using whole-cell lysates from human, mouse, or rat tissues/cells; optimize antibody dilution and blocking conditions for your specific system.
• In IF/ICC, validate permeabilization and fixation protocols to ensure proper nuclear/cytoplasmic detection of PRMT5.
• For ELISA, this pAb can be paired with a suitable capture/detection antibody (not supplied) to develop a sandwich ELISA; validate linearity and sensitivity.
• Since PRMT5 is ubiquitously expressed and conserved, cross-reactivity may extend to other species; confirm with appropriate controls.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a trusted one-stop provider of IVD raw materials, connecting diagnostic manufacturers and research laboratories with verified antibodies, antigens, and ancillary reagents. This anti-PRMT5 polyclonal antibody supports epigenetics and methylation research, and CamelBio can further supply optimized antibody pairs, bulk quantities, and rare-target sourcing for your assay development needs. Our expert team streamlines your transition from concept to clinic with reliable, validated materials and technical guidance.