Anti-PRMT5 Monoclonal Antibody for WB, IHC-P, ELISA - O14744

Core Product Specifications and Parameters

Biological Background: PRMT5 Function and Localization

• PRMT5 is a type II arginine methyltransferase that catalyzes the symmetrical dimethylation of arginine residues, with a preference for forming monomethylarginine (MMA) over symmetric dimethylarginine (sDMA) (PubMed:10531356, PubMed:11152681, PubMed:11747828). Related references: PMID:10531356, PMID:11152681, PMID:11747828
• It specifically methylates Sm proteins D1 (SNRPD1) and D3 (SNRPD3), a modification critical for the assembly and biogenesis of snRNP core particles and thereby for pre-mRNA splicing (PubMed:11747828, PubMed:12411503, PubMed:17709427). Related references: PMID:11747828, PMID:12411503, PMID:17709427
• PRMT5 acts as a transcriptional corepressor by methylating histone H4 at Arg-3 (H4R3) and histone H3 at Arg-8; it binds and represses the promoters of gamma-globin genes HBG1/HBG2 in conjunction with LYAR (PubMed:25092918). Related references: PMID:25092918
• It is localized in the cytoplasm, nucleus, chromosome, and Golgi apparatus, indicating its involvement in both nuclear (transcriptional regulation, RNA splicing) and cytoplasmic (signal transduction) processes.
• Key non-histone substrates include EGFR, RAF1, p53/TP53, POLR2A, and Piwi proteins, through which PRMT5 modulates EGF signaling, DNA damage responses, and RNA polymerase II transcription termination (PubMed:21258366, PubMed:21917714, PubMed:19011621, PubMed:26700805). Related references: PMID:21258366, PMID:21917714, PMID:19011621, PMID:26700805

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide mapping to amino acids 1-100 of human PRMT5. The antibody is expected to recognize the N-terminal region; verify the epitope if precise mapping is required.
• For Western blotting, a band of approximately 73 kDa is predicted; consider using whole-cell lysates from cell lines known to express PRMT5 (e.g., HeLa, HEK293) as positive controls.
• For immunohistochemistry (paraffin-embedded sections), standard antigen retrieval protocols (e.g., citrate buffer, pH 6.0) may be necessary; validate the staining in a relevant tissue panel.
• Due to high sequence conservation across human, mouse, and rat, the antibody is expected to cross-react; however, always validate in the species and experimental system of interest.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with one-stop access to IVD raw materials and technical services. This anti-PRMT5 rabbit monoclonal antibody is offered for research and diagnostic assay development in epigenetics, cancer biology, and transcriptional regulation. We can also supply validated antibody pairs, bulk ancillary reagents, and support rare target sourcing to accelerate your project from concept to clinic.