Anti-PRMT2 Rabbit Polyclonal Antibody for WB, ELISA - P55345

Core Product Specifications and Parameters

Biological Background: PRMT2 Function and Localization

• Protein arginine N-methyltransferase 2 (PRMT2), also known as histone-arginine N-methyltransferase PRMT2, is encoded by the PRMT2 gene (alternative names: HMT1, HRMT1L1). It belongs to the protein arginine methyltransferase family.
• Functions as an arginine methyltransferase that methylates the guanidino nitrogens of arginyl residues in proteins such as STAT3, FBL, and histone H4. Related references: PMID:19405910
• Acts as a transcriptional coactivator by interacting with nuclear receptors: it enhances androgen receptor (AR)-mediated transactivation with NCOA2, and estrogen receptor (ER)-mediated transactivation with estrogen. Also enhances PGR, PPARG, and RARA-mediated transactivation. Related references: PMID:17587566 PMID:12039952
• May inhibit NF-kappa-B transcription and promote apoptosis, represses E2F1 transcriptional activity in an RB1-dependent manner (by similarity), and is involved in C15ORF39-mediated inhibition of microglial inflammatory responses by suppressing NF-kappa-B signaling. Related references: PMID:16648481 PMID:38892217
• Subcellularly, PRMT2 localizes to the cytoplasm, nucleus, and nucleolus, indicating dynamic shuttling and diverse functional roles.
• Widely expressed across tissues, with high expression in androgen target organs such as heart, prostate, skeletal muscle, ovary, and spinal cord.
• Contains a S-adenosyl-L-methionine (SAM) binding domain for methyltransferase activity and an SH3 domain, and undergoes alternative splicing.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a region (aa 290-433) within the C-terminal portion of human PRMT2. Ensure the antibody recognizes the target epitope in your specific assay format.
• For Western blotting, PRMT2 has a predicted molecular weight of approximately 49 kDa. Consider using whole cell lysates from human cell lines such as LNCaP (prostate) or MCF-7 (breast), which express PRMT2, and validate with appropriate controls.
• In ELISA applications, optimize coating conditions and antibody dilutions; cross-reactivity with other PRMT family members has not been tested.
• This antibody has been validated for human reactivity only; cross-reactivity with other species is unknown and should be verified if needed.
• As a rabbit polyclonal antibody, batch-to-batch consistency may vary; consider lot-specific validation for critical experiments.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides a comprehensive one-stop sourcing bridge for IVD raw materials, supporting diagnostic developers from concept to clinic. For your PRMT2-related research and assay development, we offer validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents. Our platform streamlines the procurement of rare-target raw materials, ensuring timely supply for your diagnostic projects.