Anti-PRMT1 Rabbit Monoclonal Antibody for WB, IHC-P, IF/ICC, ELISA - Q99873

Core Product Specifications and Parameters

Biological Background: PRMT1 Function and Localization

• PRMT1 (Protein arginine N-methyltransferase 1) is the primary enzyme catalyzing asymmetric dimethylation of arginine residues, with histone H4 Arg-3 (H4R3me2a) being a major substrate that marks active chromatin.
  Related references:
  PMID:10749851 PMID:15741314 PMID:16879614

• It methylates a wide array of non-histone substrates, including RNA-binding proteins (FMR1, ILF3, HNRNPA1, RBM15), transcription factors (ESR1, FOXO1, TAF15), and signaling regulators (MAP3K5/ASK1, NPRL2), modulating their functions, interactions, and stability.
  Related references:
  PMID:26575292 PMID:18951090 PMID:22095282 PMID:38006878 PMID:25733715

• PRMT1 participates in the DNA damage response by methylating MRE11 and TP53BP1, which are critical for repair pathway activation.
  Related references:
  PMID:16294045 PMID:29651020

• Overexpression of PRMT1 is observed in colorectal cancer, and it promotes glioblastomagenesis via CHTOP/TET1-dependent H4R3 methylation, highlighting its role in tumorigenesis.
  Related references:
  PMID:28040436 PMID:25284789

• PRMT1 is predominantly nuclear (nucleoplasm), but also localizes to the cytoplasm, cytosol, and lysosome membrane, reflecting its diverse cellular functions.

• The enzyme is subject to regulatory post-translational modifications, including acetylation, phosphorylation, and ubiquitination.

• Multiple alternatively spliced isoforms exist, and it is widely expressed across tissues, with pronounced levels in colorectal cancer.
  Related references:
  PMID:11097842 PMID:28040436

Experimental Guidance and Technical Tips

• The immunogen spans the N-terminal region (aa 1-100) of human PRMT1, a domain that is highly conserved across human, mouse, and rat, supporting the observed cross-reactivity. Consider sequence alignment for other species.
• For Western blotting, a predominant band at ~42kDa is expected; validate in your specific experimental system and consider potential post-translational modifications that may cause minor shifts.
• For immunohistochemistry (IHC-P) and immunofluorescence (IF/ICC), optimize antigen retrieval and fixation conditions, as results may vary depending on tissue type and target abundance.
• This antibody can be used in ELISA; appropriate coating antigen and detection conditions should be empirically determined.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides integrated sourcing solutions for IVD raw materials, supporting epigenetic and cancer research with validated anti-PRMT1 antibodies, recombinant proteins, and assay development tools. We offer bulk monoclonal/polyclonal antibodies, custom antibody pair development, and rare-target sourcing to streamline your diagnostic pipeline from concept to clinic.