Anti-PRL/Prolactin Rabbit Monoclonal Antibody for IHC-P, ELISA - P01236

Core Product Specifications and Parameters

Biological Background: Prolactin Function and Localization

• Prolactin is a peptide hormone primarily synthesized in the anterior pituitary gland, with its main function being the promotion of lactation in the mammary gland.
• The mature protein is secreted into the bloodstream to act as a circulating hormone, influencing reproductive and metabolic processes.
• Prolactin undergoes co- and post-translational modifications, including cleavage of the signal peptide, formation of disulfide bonds, N-linked glycosylation, and phosphorylation, which can affect its biological activity and clearance.
• As indicated by UniProt keywords, Prolactin is classified as a hormone with roles in lactation and is a member of the somatotropin/prolactin family.
• The protein serves as a reference proteome component and has been identified through proteomics and direct protein sequencing, highlighting its clinical and research relevance.

Experimental Guidance and Technical Tips

• This antibody, generated against a recombinant Prolactin protein, is suitable for detecting both tissue-localized (IHC-P) and soluble (ELISA) forms of the antigen; experimental conditions may require optimization for each application.
• For IHC-P, consider testing standard antigen retrieval methods (e.g., heat-induced epitope retrieval in citrate or EDTA buffer) to enhance signal in formalin-fixed, paraffin-embedded tissues.
• In ELISA, the antibody may be employed as a capture or detection reagent in sandwich immunoassays; validate with appropriate recombinant or native Prolactin standards in the relevant sample matrix (e.g., serum, cell supernatant).
• Cross-reactivity data indicate specificity for human Prolactin; when working with other species, verify sequence homology and perform preliminary binding tests if interdisciplinary studies are planned.
• To confirm band identity in Western blot (if applied beyond listed applications), the predicted molecular weight of Prolactin is ~26 kDa; use positive control lysates from human pituitary gland or recombinant protein.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio connects diagnostic manufacturers, laboratories, and research institutes with a comprehensive portfolio of IVD raw materials, including this anti-human Prolactin monoclonal antibody for lactation and endocrine studies. We streamline procurement with validated antibody pairs, optimized recombinant proteins, and bulk ancillary reagents, and we specialize in sourcing rare-target raw materials to support your assay development from concept to clinic.