Anti-PRL Rabbit Polyclonal Antibody for WB, IHC-P, ELISA - P01236

Core Product Specifications and Parameters

Biological Background: PRL Function and Localization

• Prolactin (PRL) is a secreted hormone that primarily acts on the mammary gland to promote lactation.
• The mature protein contains disulfide bonds that stabilize its three-dimensional structure, and it is both N-glycosylated and phosphorylated.
• Consistent with its endocrine role, PRL is released into the extracellular space and circulates in the blood.
• Its molecular weight is approximately 26 kDa (UniProt: 25,876 Da), and the full-length structure has been determined by X-ray crystallography.
• Post-translational modifications, including glycosylation and phosphorylation, may modulate hormone activity and receptor binding.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 29–227 of human PRL, which covers the majority of the mature peptide. The resulting polyclonal antibody is expected to recognize the secreted form of prolactin.
• For Western blotting, use reducing sample buffer to resolve disulfide bonds. A band at ~26 kDa is anticipated; minor mobility shifts due to glycosylation variants may be observed.
• In immunohistochemistry (IHC-P), validate antibody staining with appropriate positive and negative control tissues. Consider optimizing antigen retrieval and detection protocols.
• For ELISA, pair with a suitable detection antibody and calibrate against recombinant or purified PRL standards.
• Cross-reactivity with mouse and rat PRL is listed; independent verification in your model system is recommended.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio connects diagnostic manufacturers and researchers with high-quality IVD raw materials. This anti-PRL polyclonal antibody supports lactation and hormone research, and we can supply validated antibody pairs, bulk quantities, and ancillary reagents to accelerate your assay development. Reach out for customized sourcing and technical consulting.