Anti-PRKRIP1 Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - Q9H875

Core Product Specifications and Parameters

Biological Background: PRKRIP1 Function and Localization

• Protein identity and aliases: PRKRIP1 (PRKR-interacting protein 1), also designated C114, KRBOX3, is a 21 kDa nuclear protein encoded by the PRKRIP1 gene (GeneID: 79706) in humans, with orthologs conserved across species.
• Core splicing function: PRKRIP1 is required for pre-mRNA splicing as an integral component of the spliceosome, the macromolecular machinery responsible for intron removal and exon ligation. Related references: PMID:28502770 PMID:30705154
• RNA binding: It binds double-stranded RNA, a feature potentially linking it to substrate recognition or regulatory interactions during splicing.
• Kinase inhibition: PRKRIP1 inhibits the kinase activity of EIF2AK2 (PKR), a protein involved in antiviral responses and stress signaling, although this inhibition has been inferred from sequence similarity.
• Subcellular localization: The protein localizes predominantly to the nucleus, with enrichment observed in the nucleolus, consistent with its roles in RNA processing.
• Alternative splicing and structural diversity: PRKRIP1 undergoes alternative splicing, generating multiple isoforms; its sequence includes predicted coiled-coil domains that may mediate protein-protein interactions.
• Spliceosomal classification: UniProt keywords classify PRKRIP1 under spliceosome, mRNA processing, and mRNA splicing, underscoring its essential participation in constitutive and possibly alternative splicing regulation.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 67–134 of human PRKRIP1, a region that may be conserved across species; consider verifying sequence identity in mouse and rat to anticipate cross-reactivity.
• For Western blot (WB), a band around 21 kDa is expected; you may need to optimize blocking and antibody dilution for individual sample types.
• In immunofluorescence/immunocytochemistry (IF/ICC), permeabilization conditions should be validated, and nuclear staining patterns (including nucleolar enrichment) may serve as a positive localization control.
• When using ELISA, include appropriate controls and consider antigen coating strategies to ensure accurate detection.
• Due to the polyclonal nature of this antibody, lot-to-lot consistency in applications like WB and IF should be confirmed in preliminary experiments.

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CamelBio streamlines the procurement of IVD raw materials, offering validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and sourcing for rare targets like PRKRIP1. Whether you are developing assays for spliceosome-related biomarkers or exploring pre-mRNA splicing mechanisms, we provide end-to-end support to accelerate your diagnostic and research programs. Contact our team to discuss customized sourcing solutions tailored to your projects.