Anti-PRKRA/PACT Rabbit Polyclonal Antibody for WB, ELISA - O75569

Core Product Specifications and Parameters

Biological Background: PRKRA/PACT Function and Localization

• PRKRA (also known as PACT, RAX) is an interferon-inducible double-stranded RNA-dependent protein kinase activator A.
• It activates EIF2AK2/PKR in the absence of dsRNA, leading to phosphorylation of EIF2S1/EFI2-alpha and subsequent inhibition of translation and induction of apoptosis.
• PRKRA is essential for siRNA production by DICER1 and for siRNA-mediated post-transcriptional gene silencing, but not for pre-miRNA processing.
• It promotes UBC9-p53/TP53 association and sumoylation, as well as phosphorylation of p53/TP53 at Lys-386 and Ser-392, enhancing p53 activity in an EIF2AK2/PKR-dependent manner.
• PRKRA localizes predominantly to the cytoplasm and the perinuclear region.
• The protein may also function as a regulator of gastric epithelial differentiation.
• Keywords associated with PRKRA include alternative splicing, disease variants (dystonia, Parkinsonism), host-virus interaction, and RNA-binding, indicating its involvement in diverse cellular processes.

Experimental Guidance and Technical Tips

• When performing Western blotting, expect a band near 34kDa corresponding to PRKRA/PACT. Since the antibody is polyclonal, it may detect multiple isoforms; validate specificity with siRNA knockdown or knockout controls.
• The antibody is suitable for ELISA applications; consider optimizing coating antigen and detection conditions.
• Cross-reactivity with mouse is reported, making it potentially useful for mouse model studies; however, confirm reactivity in your specific experimental system.
• Given PRKRA's role in RNA interference and apoptosis, consider functional studies to correlate expression changes with target pathway activation.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with streamlined access to high-quality IVD raw materials, including validated antibodies for key targets like PRKRA/PACT involved in innate immunity and gene silencing. Beyond single-antibody supply, we offer optimized monoclonal/polyclonal antibody pairs, bulk ancillary reagents, and specialized sourcing for rare targets to accelerate your assay development from concept to clinic.