Anti-PRKG2 Polyclonal Antibody for WB, IF/ICC, IP, ELISA - Q13237

Core Product Specifications and Parameters

Biological Background: PRKG2 Function and Localization

• cGMP-dependent protein kinase 2 (cGKII) is a serine/threonine-protein kinase encoded by the PRKG2 gene; alternative splicing generates multiple isoforms.
• Acts as a crucial regulator of intestinal secretion by phosphorylating and activating CFTR on the plasma membrane, mediating cGMP-dependent translocation of CFTR in jejunum. Related references: PMID:33106379
• In neurons, acts downstream of NMDAR to promote plasma membrane accumulation of GRIA1/GLUR1 at synapses, enhancing synaptic plasticity by phosphorylating GRIA1 at Ser-863 (by similarity).
• In mechanically stimulated osteoblasts, activates the MAPK/ERK pathway (MAPK3/ERK1 and MAPK1/ERK2), inducing FOS family transcription factors critical for the anabolic bone response to mechanical stimulation (by similarity).
• Predominantly localized at the apical cell membrane.
• Highly expressed in brain, lung, and intestinal mucosa.
• Contains cGMP-binding and ATP-binding domains; classified as a serine/threonine-protein kinase; associated with disease variants linked to dwarfism.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal region (aa 1-160); it may detect full-length PRKG2 or N-terminal-containing isoforms.
• For Western blot (WB), use denaturing conditions; the predicted molecular weight is approximately 87 kDa.
• For immunofluorescence/immunocytochemistry (IF/ICC), the antibody may label apical membranes; validate in intestinal or renal epithelial cell models.
• In immunoprecipitation (IP) and ELISA, include appropriate controls and optimize buffer conditions for your sample type.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a comprehensive provider of IVD raw materials, offering validated antibodies like anti-PRKG2 to facilitate research in areas such as intestinal secretion, bone growth, and synaptic plasticity. With capabilities in supplying antibody pairs, bulk ancillary reagents, and rare target sourcing, we support your diagnostic development from early stages to clinical application. Inquire today for your cGKII/PRKG2 reagent needs.