Anti-PRKG1 Polyclonal Antibody for WB and ELISA - Q13976

Core Product Specifications and Parameters

Biological Background: PRKG1 Function and Localization

• PRKG1 (cGMP-dependent protein kinase 1, also known as cGKI or PKG1) is a serine/threonine protein kinase that acts as a key mediator of nitric oxide (NO)/cGMP signaling.
• It is activated by cGMP binding and phosphorylates numerous cellular proteins to regulate platelet activation, smooth muscle contraction, cardiac function, gene expression, and CNS processes such as axon guidance and learning.
• The protein exists in multiple isoforms (e.g., cGKI-alpha and cGKI-beta) generated by alternative splicing, which may exhibit distinct tissue distributions and functions.
• Subcellularly, PRKG1 localizes primarily to the cytoplasm.
• It plays a critical role in smooth muscle relaxation by lowering intracellular calcium levels through phosphorylation of targets like IRAG1 and KCNMA1 (BKCa channels).
• PRKG1 phosphorylates RHOA, leading to vasorelaxation and reduced smooth muscle contraction.
• According to UniProt, it is primarily expressed in lung and placenta tissues.
• Key post-translational modifications include acetylation and disulfide bond formation, and the protein features allosteric cGMP-binding domains and an ATP-binding kinase domain.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 1-300 of human PRKG1, targeting the N-terminal region, which may recognize multiple isoforms.
• For Western blot, a band around 76 kDa is expected based on the calculated molecular weight.
• Consider using appropriate positive control lysates from human or mouse lung, placenta, or smooth muscle tissues where PRKG1 is expressed.
• As a polyclonal antibody, it may require careful titration to minimize non-specific binding in both WB and ELISA applications.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a dedicated one-stop sourcing partner for IVD manufacturers, offering reliable access to validated raw materials including polyclonal and monoclonal antibodies, optimized antibody pairs, and bulk ancillary reagents. This anti-PRKG1 antibody supports research into NO/cGMP signaling, smooth muscle function, and platelet biology. Through strategic supplier networks, CamelBio fulfills rare-target antibody requests and provides end-to-end technical consultation to accelerate assay development.