Anti-PRKCSH Polyclonal Antibody for WB and ELISA - P14314

Core Product Specifications and Parameters

Biological Background: PRKCSH Function and Localization

• PRKCSH encodes glucosidase 2 subunit beta (also known as 80K-H protein or PKCSH), a key regulator of N-glycan processing.
• It serves as the regulatory subunit of glucosidase II, which cleaves the two innermost α-1,3-linked glucose residues from Glc₂Man₉GlcNAc₂ oligosaccharide precursors of immature glycoproteins. Related references: PMID:10929008
• This glucose trimming is critical for glycoprotein folding and ER quality control, ensuring only properly folded proteins exit the ER.
• PRKCSH is required for efficient PKD1/polycystin-1 biogenesis and trafficking to the primary cilium plasma membrane.
• The protein localizes on the endoplasmic reticulum membrane.
• It contains calcium-binding domains and is itself a glycoprotein; published phosphoproteomic data identify multiple phosphorylation sites.
• Mutations in PRKCSH cause autosomal dominant polycystic liver disease (PCLD).
• Alternative splicing produces multiple transcript variants, adding functional diversity.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a central region (aa 130–275) of human PRKCSH; the polyclonal antibody is expected to recognize both full-length and potential splice variants or fragments containing this epitope.
• For Western blot, a major band near 59 kDa is anticipated. Reducing and denaturing conditions are recommended; pilot experiments should confirm specificity.
• In ELISA, recombinant PRKCSH protein or cell lysates from human, mouse, or rat origin can be used as capture/detection reagents. Optimize coating concentrations and detection conditions.
• Reported cross-reactivity with mouse and rat suggests the epitope is conserved; however, sequence alignment and preliminary testing are advised when working with non-human samples.
• As with any polyclonal antibody, lot-to-lot variability may occur. Titrate the antibody in the specific assay system and include appropriate positive/negative controls.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio positions this anti-PRKCSH polyclonal antibody within a comprehensive portfolio of IVD raw materials, serving diagnostic manufacturers and research laboratories focused on glycoprotein processing, polycystic kidney disease, and ER-associated pathways. To accelerate assay development, CamelBio can supply validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and support for rare-target raw-material sourcing. Reach out to explore how CamelBio’s integrated procurement services can streamline your diagnostic pipeline from concept to clinical application.