Anti-PRKAR2B Rabbit Monoclonal Antibody for WB, IHC-P, ELISA - P31323

Core Product Specifications and Parameters

Biological Background: PRKAR2B Function and Localization

• PRKAR2B encodes the type II-beta regulatory subunit of cAMP-dependent protein kinase (PKA), a key enzyme in cAMP signaling pathways.
• This regulatory subunit binds cAMP, and upon binding, it releases the catalytic subunits, leading to PKA activation and phosphorylation of downstream targets.
• Type II regulatory chains mediate membrane association by binding to anchoring proteins, including MAP2 kinase, thereby localizing PKA activity to specific subcellular compartments.
• The protein is predominantly localized to the cytoplasm and also associates with the cell membrane.
• Tissue expression of PRKAR2B is variable; it represents one of four regulatory subunit types (I-alpha, I-beta, II-alpha, II-beta) that exhibit tissue-specific expression patterns.
• PRKAR2B contains two cAMP-binding domains and is regulated by phosphorylation (identified as a phosphoprotein in proteomics studies).
• Keywords from UniProt: cAMP, cAMP-binding, cell membrane, cytoplasm, membrane, nucleotide-binding, phosphoprotein, proteomics identification, reference proteome, repeat.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide spanning amino acids 319–418, which corresponds to a region near the C-terminus of PRKAR2B. Consider verifying antibody specificity using peptide competition assays or by testing in PRKAR2B knockout/knockdown cell lysates.
• For Western blotting, an expected band at approximately 46 kDa (consistent with the calculated molecular weight of 46,302 Da) should be detected. Include appropriate loading controls and confirm band identity with relevant biological samples.
• In immunohistochemistry (paraffin-embedded, IHC-P), antigen retrieval conditions may need optimization; test citrate or EDTA-based protocols on known positive control tissues.
• For ELISA applications, the peptide-based immunogen suggests the antibody may recognize linear epitopes; a direct or sandwich ELISA format can be evaluated.
• Cross-reactivity with mouse and rat PRKAR2B has been reported; validate in the relevant sample system to ensure consistent detection across species.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your trusted one-stop partner for IVD raw-material sourcing, offering a comprehensive portfolio of validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and custom sourcing for rare targets. For research and diagnostic development in cAMP signaling and kinase pathways, the PRKAR2B rabbit monoclonal antibody provides a reliable tool, backed by CamelBio's commitment to supporting every stage from concept to clinic.