Anti-PRKAG3 Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - Q9UGI9

Core Product Specifications and Parameters

Biological Background: PRKAG3 Function and Localization

• PRKAG3 encodes the gamma-3 regulatory subunit of AMP-activated protein kinase (AMPK), an energy sensor that maintains cellular energy homeostasis by binding AMP, ADP, and ATP. Related references: PMID:14722619, PMID:17878938, PMID:24563466

• In response to low intracellular ATP, AMPK activation promotes catabolic pathways and inhibits anabolic processes, including protein, carbohydrate, and lipid biosynthesis, as well as cell growth and proliferation. Related references: PMID:14722619, PMID:17878938, PMID:24563466

• PRKAG3 is predominantly expressed in skeletal muscle, with weak expression in heart and pancreas, reflecting its specialized role in muscle energy metabolism.

• The subunit contains CBS domains that mediate binding to adenosine nucleotides; AMP binding allosterically activates the catalytic alpha subunit, while ATP promotes dephosphorylation and inactivation. Related references: PMID:14722619, PMID:17878938, PMID:24563466

• Key biological processes linked to PRKAG3 include fatty acid biosynthesis, fatty acid metabolism, lipid biosynthesis, and lipid metabolism, underscoring its broad metabolic regulatory functions.

• The protein undergoes alternative splicing and post-translational modifications, such as phosphorylation and glycosylation, as identified in proteomics studies.

Experimental Guidance and Technical Tips

• For Western blot (WB), the predicted molecular weight is 54kDa; consider using skeletal muscle lysates as a positive control, given the tissue-specific expression of PRKAG3.
• For immunocytochemistry/immunofluorescence (IF/ICC), validate fixation and permeabilization conditions in cultured muscle cell lines to optimize signal detection.
• When using ELISA, ensure the antigen coating strategy aligns with the immunogen region (amino acids 1-70 of human PRKAG3).
• Cross-reactivity with mouse and rat PRKAG3 is expected, but verify signal specificity in the relevant species’ tissue or cell extracts.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive sourcing partner for IVD raw materials, offering this anti-PRKAG3 polyclonal antibody to support research into cellular energy metabolism and muscle physiology. Alongside this product, we supply validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and specialized sourcing for rare targets, helping diagnostic developers streamline assay workflows from concept to clinic.