Anti-PRKAG1 Rabbit Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - P54619

Core Product Specifications and Parameters

Biological Background: PRKAG1 Function and Localization

• PRKAG1 encodes the 5'-AMP-activated protein kinase subunit gamma-1, the regulatory gamma subunit of the AMPK heterotrimer, which acts as a cellular energy sensor.
• Acts as the AMP/ATP-binding subunit of AMPK, an energy sensor protein kinase that regulates cellular energy metabolism by activating energy-producing pathways and inhibiting energy-consuming processes. Related references: PMID:21680840 PMID:24563466
• AMP binding to PRKAG1 leads to allosteric activation, phosphorylation, and prevention of dephosphorylation of the catalytic alpha subunit (PRKAA1/PRKAA2), while ATP promotes dephosphorylation and inactivation of AMPK. Related references: PMID:21680840 PMID:24563466
• Involved in the inhibition of fatty acid, cholesterol, and lipid biosynthesis under low intracellular ATP conditions, contributing to metabolic regulation. Related references: PMID:21680840 PMID:24563466
• Also regulates cellular polarity by remodeling the actin cytoskeleton, probably through indirect activation of myosin. Related references: PMID:21680840 PMID:24563466
• Contains two cystathionine-beta-synthase (CBS) domains that form the nucleotide-binding pockets for AMP, ADP, and ATP.
• Classified as a glycoprotein and phosphoprotein and reported to undergo alternative splicing.

Experimental Guidance and Technical Tips

• The immunogen spans the full-length human PRKAG1 (aa 1-331), which may detect all known isoforms; verify in the relevant sample system.
• For Western blot, an approximate band at 38 kDa is expected; use appropriate controls and consider validation in target tissues.
• For IHC-P and IF/ICC, optimization of antigen retrieval and blocking conditions may be necessary; include positive and negative controls.
• In ELISA, this antibody can be used for quantitative detection of PRKAG1; confirm linearity and sensitivity in your assay setup.
• Cross-reactivity with mouse and rat orthologs allows translation of findings to common rodent models; validate expression levels in the chosen model.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your comprehensive partner for IVD raw-material sourcing, offering access to validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and rare target raw materials. For PRKAG1 and metabolic disease research, we facilitate streamlined procurement from concept to clinic, helping you secure the precise reagents your diagnostic assays demand. Rely on CamelBio to bridge your sourcing gaps and accelerate development cycles.