Anti-PRKACG Rabbit Polyclonal Antibody for WB & ELISA - P22612

Core Product Specifications and Parameters

Biological Background: PRKACG Function and Localization

• PRKACG encodes the gamma catalytic subunit of cAMP-dependent protein kinase (PKA), a serine/threonine-protein kinase essential for cAMP-mediated signaling.
• The enzyme phosphorylates a large number of substrates in the cytoplasm and nucleus, regulating diverse cellular processes.
• It belongs to the protein kinase superfamily, featuring conserved ATP-binding and catalytic domains characteristic of serine/threonine kinases.
• Expression is predominantly testis-specific, indicating a specialized role in male germ cell function; however, other tissues like brain and ovary have not been thoroughly evaluated.
• Post-translational modifications include N-terminal myristoylation, which may facilitate membrane association, and regulatory phosphorylation.
• Mutations in PRKACG are associated with platelet-type bleeding disorder 19 (BDPLT19), underscoring its role in hemostasis.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide derived from amino acids 1-351 of human PRKACG; the antibody is expected to recognize the full-length protein and possibly truncated variants.
• For Western blotting, use reducing conditions and expect a band near 40 kDa; include positive controls such as human testis lysate or cells overexpressing PRKACG.
• Polyclonal antibodies may exhibit background; optimize primary antibody dilution (e.g., 1:500-1:2000) and blocking buffer for your specific sample.
• In ELISA applications, validate detection sensitivity using purified recombinant PRKACG protein or the immunizing peptide as standard.
• Cross-reactivity with mouse and rat proteins is indicated; however, confirm by sequence alignment and test in relevant tissue lysates before interpreting results.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio streamlines IVD raw-material procurement, offering a comprehensive portfolio including validated antibody pairs, optimized polyclonal antibodies, and ancillary reagents. This anti-PRKACG antibody supports research in kinase signaling and reproductive biology, with flexible supply options for diagnostic kit development. For custom or rare targets, our technical and supply chain experts provide end-to-end consulting to accelerate your project from bench to clinic.