Anti-PRKAB2 Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - O43741

Core Product Specifications and Parameters

Biological Background: PRKAB2 Function and Localization

• PRKAB2 encodes the non-catalytic beta-2 subunit of AMP-activated protein kinase (AMPK), a central energy sensor that maintains cellular energy homeostasis.
• The beta subunit acts as a scaffold, bridging the catalytic alpha subunit (PRKAA1/2) and regulatory gamma subunit (PRKAG1/2/3) to form the functional AMPK heterotrimeric complex.
• AMPK activation in response to low intracellular ATP levels phosphorylates key metabolic enzymes and transcription factors, inhibiting anabolic processes such as protein, carbohydrate, and lipid biosynthesis while promoting catabolic pathways.
• Beyond metabolism, PRKAB2 contributes to regulation of cellular polarity by remodeling the actin cytoskeleton, likely through indirect activation of myosin.
• The protein is annotated with keywords including alternative splicing, fatty acid biosynthesis and metabolism, lipid biosynthesis and metabolism, and phosphoprotein, highlighting its dynamic regulation and metabolic roles.
• Multiple isoforms exist due to alternative splicing, and the canonical protein has a molecular weight of approximately 30 kDa.

Experimental Guidance and Technical Tips

• The immunogen covers the N-terminal region (amino acids 1–272), so the antibody may recognize full-length PRKAB2 and N-terminal fragments; cross-reactivity with other isoforms should be validated in the relevant sample system.
• For western blotting, a band around 30 kDa is expected; consider using a 10% or 12% SDS-PAGE gel for optimal resolution.
• In immunofluorescence/ICC, nuclear and cytoplasmic localization may be observed depending on cell type and metabolic state; co-staining with organelle markers can aid interpretation.
• ELISA sensitivity and specificity should be confirmed using recombinant protein or cell lysates from appropriate species (human, mouse, rat) as per the antibody's reported cross-reactivity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as your comprehensive partner for IVD raw-material sourcing, providing high-quality antibodies like this anti-PRKAB2 polyclonal antibody to support metabolic and energy research applications. Our integrated supply chain delivers validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and custom sourcing for rare targets, all backed by technical consulting from concept to clinic. Let CamelBio streamline your raw-material procurement so you can focus on advancing your diagnostic development.