Anti-PRK2/PKN2 Rabbit Polyclonal Antibody for WB and ELISA - Q16513

Core Product Specifications and Parameters

Biological Background: PRK2/PKN2 Function and Localization

• Encodes serine/threonine-protein kinase N2 (PKN2), also known as PKN gamma, protein kinase C-like 2, and protein-kinase C-related kinase 2; a member of the PKC-related kinase family.
• Acts as a Rho/Rac effector kinase involved in diverse signal transduction pathways, including regulation of cell cycle progression, actin cytoskeleton assembly, cell migration, cell adhesion, and transcriptional activation.
• Localizes to multiple subcellular compartments: cytoplasm, nucleus, membrane, lamellipodium, cleavage furrow, midbody, and cell junctions, consistent with its roles in cytokinesis and adhesion.
• Exhibits ubiquitous expression, with notable expression in various tumor cell lines, particularly in bladder tumor cells.
• Phosphorylates HDAC5, impairing its nuclear import; phosphorylates CTTN, reducing its actin-binding capacity; and stimulates FYN kinase activity during keratinocyte differentiation.
• Involved in negative regulation of ciliogenesis (PubMed:27104747). Related references: PMID:27104747
• During microbial infection, phosphorylates HCV NS5B, stimulating HCV RNA replication.
• Key post‑translational modifications include phosphorylation and acetylation; the protein contains a coiled‑coil domain and belongs to the kinase superfamily.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 600–700 near the C‑terminus; the antibody may recognize full‑length (~112 kDa) and potential cleavage products. Validate in your target sample system.
• For Western blot, a predominant band around 112 kDa is expected. Optimize antibody dilution (e.g., 1:500–1:2000) and blocking conditions empirically.
• For ELISA, perform a titration series to determine the optimal capture/detection antibody concentration for your antigen format.
• As a polyclonal reagent, lot‑to‑lot variation may occur; verify each new lot with appropriate positive and negative controls.
• Cross‑reactivity with mouse and rat is reported; if using these species, confirm specificity in your experimental context.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers and research laboratories with a comprehensive portfolio of IVD raw materials, including validated polyclonal antibodies like this anti-PRK2/PKN2 antibody for WB and ELISA applications. Whether your project involves kinase signaling, cell cycle studies, or cancer biomarker development, we provide essential raw materials such as antibody pairs, bulk ancillary reagents, and rare‑target sourcing to streamline your workflow from concept to clinic.