Anti-Prion Protein Polyclonal Antibody for WB, ELISA - P04156

Core Product Specifications and Parameters

Biological Background: Prion Protein Function and Localization

• The major prion protein is encoded by the PRNP gene and is also known as ASCR, PrP27-30, PrP33-35C, CD230, and p27-30.
• Its physiological function is not fully understood; it may contribute to neuronal development and synaptic plasticity, and is potentially required for myelin sheath maintenance.
• PrP may promote myelin homeostasis by acting as an agonist for the ADGRG6 receptor.
• It has been implicated in cellular iron uptake and iron homeostasis.
• In vitro studies indicate that soluble oligomers of PrP are toxic to cultured neuroblastoma cells and can induce apoptosis.
• The protein localizes to the cell membrane and the Golgi apparatus, anchored by a GPI moiety.
• PrP is a glycoprotein with a disulfide bond and binds copper and zinc ions; it may provide these metals for the ascorbate-mediated degradation of heparan sulfate chains on GPC1.
• Post-translational modifications and metal-binding properties are key features of this prion protein.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 23-230 of human prion protein (NP_001073592.1), covering the N-terminal domain. This antibody may recognize both full-length and truncated forms of PrP.
• In Western blotting, the predicted molecular weight is approximately 28 kDa, but glycation and GPI anchor modifications can cause bands to appear at different sizes. Validate the observed band against appropriate controls.
• For ELISA applications, consider using this polyclonal antibody as a capture or detection reagent, paired with a monoclonal antibody to improve specificity.
• As the antibody cross-reacts with mouse PrP, it can be employed in mouse model studies of prion diseases; however, confirm cross-reactivity in your specific system.
• Optimal dilutions and incubation conditions should be determined empirically for each experimental setup.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with a comprehensive one-stop platform for IVD raw materials, technical services, and consulting. For prion protein research and neurodegenerative disease diagnostics, we offer validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and sourcing of rare targets to support your assay development from concept to clinic. Partner with CamelBio to streamline your raw material procurement and accelerate your project timelines.