Anti-Prion Protein (CD230/PrPc) Rabbit Polyclonal Antibody for WB, ELISA - P04156

Core Product Specifications and Parameters

Biological Background: Prion Protein Function and Localization

• Major prion protein (PrP), encoded by the PRNP gene, is a membrane‑anchored glycoprotein and the precursor of the infectious prion agent; alternative names include CD230, PrPc, PrP27‑30, and PrP33‑35C.
• Its primary physiological function remains elusive, but it has been implicated in neuronal development, synaptic plasticity, and maintenance of the myelin sheath.
• PrP may promote myelin homeostasis by serving as an agonist for the adhesion G‑protein coupled receptor ADGRG6.
• It has been proposed to participate in cellular iron uptake and homeostasis, and soluble oligomers of PrP are cytotoxic to neuroblastoma cells in vitro, inducing apoptosis (By similarity).
• PrP binds GPC1 via its heparan sulfate chains, directing the complex to lipid rafts, and supplies Cu²⁺ or Zn²⁺ ions necessary for ascorbate‑driven GPC1 deaminase‑mediated degradation of heparan sulfate side chains.
• The protein localizes to the cell membrane and the Golgi apparatus via a C‑terminal glycosylphosphatidylinositol (GPI) anchor; it contains an octarepeat region that binds copper and zinc.
• Mutations in PRNP cause familial neurodegenerative disorders, including Creutzfeldt‑Jakob disease (CJD), Gerstmann‑Sträussler‑Scheinker syndrome (GSS), and fatal familial insomnia (FFI), underscoring its pathophysiological importance.

Experimental Guidance and Technical Tips

• The immunogen spans amino acids 23–230, encompassing the mature PrP sequence that includes the octarepeat region and the globular domain; this design should detect both full‑length and truncated isoforms.
• For Western blot, the predicted molecular weight is 28 kDa, but extensive glycosylation often results in bands in the 33–40 kDa range; consider using deglycosylating enzymes if precise molecular weight confirmation is required.
• Cross‑reactivity with mouse PrP is confirmed; therefore, the antibody may be suitable for studies in mouse models, but validation in the relevant sample system is recommended.
• In ELISA, recombinant PrP protein can serve as a positive control; ensure optimal coating conditions and antibody dilutions are determined empirically.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers and research laboratories streamlined access to premium IVD raw materials, technical services, and consulting from concept to clinic. This anti‑Prion Protein polyclonal antibody is part of a broader portfolio that includes validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and custom sourcing for rare targets. For reliable support in developing neurodegenerative disease assays, CamelBio stands as your dedicated sourcing partner.