Anti-Presenilin 1 Rabbit Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - P49768

Core Product Specifications and Parameters

Biological Background: Presenilin 1 Function and Localization

• Catalytic subunit of the gamma-secretase complex, an endoprotease that cleaves integral membrane proteins such as APP and Notch receptors; requires other gamma-secretase components for activity. Related references: PMID:10206644, PMID:10545183, PMID:10593990, PMID:15274632, PMID:25043039, PMID:26280335
• Plays a key role in Notch and Wnt signaling pathways by processing regulatory proteins and modulating cytoplasmic beta-catenin (CTNNB1) levels. Related references: PMID:10593990, PMID:10811883, PMID:10899933
• Stimulates cell-cell adhesion through interaction with CDH1 (E-cadherin); under apoptosis or calcium influx, cleaves CDH1, leading to disassembly of cadherin/catenin complexes and negative regulation of Wnt signaling. Related references: PMID:11953314, PMID:9738936
• Mediates the proteolytic cleavage of EphB2 receptor, generating a cytoplasmic fragment (EphB2/CTF2) involved in downstream signaling. Related references: PMID:17428795, PMID:28269784
• The holoprotein functions as a calcium-leak channel, allowing passive calcium movement from the endoplasmic reticulum to the cytosol, contributing to calcium homeostasis. Related references: PMID:16959576, PMID:25394380
• Regulates neurite outgrowth and is involved in presynaptic facilitation, spike transmission, and synaptic vesicle replenishment via gamma-secretase-dependent control of SYT7 presynaptic expression. Related references: PMID:15004326, PMID:20460383
• Subcellularly localizes to the endoplasmic reticulum, Golgi apparatus, cell membrane, early endosomes, growth cones, axons, and synapses.
• Widely expressed in tissues including brain, liver, spleen, and lymph nodes; also detected in neutrophil azurophilic granules and platelet cytoplasmic granules. Related references: PMID:11987239, PMID:7596406, PMID:8574969, PMID:8641442

Experimental Guidance and Technical Tips

• The immunogen covers the intracellular loop region (amino acids 290–380) of human Presenilin 1; consider using antigen retrieval protocols for IHC-P on formalin-fixed paraffin-embedded sections.
• For western blot, the predicted molecular weight is 53 kDa, but full-length PSEN1 undergoes endoproteolytic cleavage into ~30 kDa N-terminal and ~20 kDa C-terminal fragments; observe both full-length and processed forms depending on sample preparation.
• When performing immunofluorescence or ICC, permeabilize cells to allow antibody access to the intracellular epitope; confirm ER/Golgi and cell membrane localization.
• Perform a dilution series to determine the optimal working concentration for your specific assay and sample type.
• Validate cross-reactivity in mouse and rat samples, as indicated by the supplier’s data.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio connects diagnostic manufacturers, laboratories, and research institutes with validated antibodies and raw materials for IVD development, including this anti-Presenilin 1 polyclonal antibody for neurodegeneration and Alzheimer’s disease research. Our comprehensive portfolio provides monoclonal/polyclonal antibodies, optimized antibody pairs, and bulk ancillary reagents, simplifying procurement from a single B2B partner. For rare-target or custom raw-material sourcing, our technical consulting team supports end-to-end project needs, helping you move from concept to clinic.