Anti-PREPL Polyclonal Antibody for WB, IF/ICC, ELISA - Q4J6C6

Core Product Specifications and Parameters

Biological Background: PREPL Function and Localization

• Prolyl endopeptidase-like (PREPL) is a serine peptidase encoded by the PREPL gene (also known as CMS22 or KIAA0436).
• Serine peptidase with unclear substrate specificity; it does not cleave peptides after arginine or lysine residues. Related references: PMID:16143824, PMID:16385448, PMID:28726805
• Regulates trans-Golgi network morphology and protein sorting by modulating the membrane binding of the AP-1 adaptor complex. Related references: PMID:23321636
• May play a role in the regulation of synaptic vesicle exocytosis. Related references: PMID:24610330
• Subcellular localization includes cytoplasm, cytosol, trans-Golgi network, cytoskeleton, Golgi apparatus, and nucleus.
• Widely expressed, with higher levels in brain, skeletal muscle, heart, and kidney; detected in pyramidal neurons and at neuromuscular junction endplates.
• Subject to acetylation and alternative splicing; variants are associated with congenital myasthenic syndrome.
• Predicted molecular weight is approximately 84 kDa (UniProt: 83927 Da).

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 578–727 in the C‑terminal region of human PREPL. Consider that the antibody may recognize epitopes within this segment.
• For western blot, a band near 84 kDa is expected; validate detection in relevant lysates (e.g., brain, muscle, or kidney).
• In immunofluorescence/immunocytochemistry, take into account the reported localization to cytoplasm, Golgi, and nucleus; optimize fixation and permeabilization conditions accordingly.
• As a polyclonal reagent, performance may vary between lots. Include appropriate positive and negative controls and consider consistency checks across production lots for critical applications.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio streamlines IVD raw‑material procurement with a comprehensive portfolio that includes validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents. Given PREPL’s involvement in Golgi trafficking and neuromuscular function, our sourcing expertise supports assay development for neurodegenerative and neurodevelopmental research areas. We also specialize in rare‑target sourcing and scalable supply solutions, helping diagnostic manufacturers and labs move efficiently from concept to clinic.