Anti-PRELID3B Rabbit Polyclonal Antibody for WB, ELISA - Q9Y3B1

Core Product Specifications and Parameters

Biological Background: PRELID3B Function and Localization

• PRELID3B, also known as SLMO2, is a PRELI domain-containing protein encoded by the PRELID3B (C20orf45) gene on human chromosome 20.
• The protein is annotated as a phosphoprotein based on large-scale proteomics identification, suggesting potential post-translational regulation by phosphorylation.
• Alternative splicing of PRELID3B produces multiple transcript variants, which may expand functional diversity.
• The three-dimensional structure of PRELID3B has been experimentally determined, providing a molecular framework for future structure-function studies.
• While specific functional annotation is currently absent from UniProt, PRELI domain family members are often implicated in lipid-binding and mitochondrial-related processes; the exact role of PRELID3B remains to be characterized.
• The protein has a calculated molecular weight of approximately 21.5 kDa and is classified as a reference proteome component.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a synthetic peptide from amino acids 80–180 of human PRELID3B; antibody reactivity is expected to recognize linear epitopes within this region, making it suitable for ELISA against peptide or denatured protein.
• For Western blotting, the predicted molecular weight is ~21 kDa. Use freshly prepared samples and protease/phosphatase inhibitors to preserve the phosphoprotein status. Antibody specificity should be confirmed with a blocking peptide or knockdown control.
• Consider testing antibody reactivity in human cell lines expressing PRELID3B, as cross-reactivity with other species has not been characterized.
• Optimize blocking and antibody incubation conditions according to your standard protocols; non-specific bands may be reduced by using 5% non-fat milk or BSA.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a trusted sourcing partner for IVD raw materials, offering a streamlined channel to validated antibodies, bulk ancillary reagents, and technical support for diagnostic developers and researchers. This anti-PRELID3B polyclonal antibody complements our portfolio for targets involved in signal transduction and potential mitochondrial biology. Whether you require optimized monoclonal/polyclonal antibodies, validated antibody pairs, or rare target sourcing, CamelBio’s supply chain expertise helps accelerate your project from concept to clinic.