Anti-PRELID3A Polyclonal Antibody for IHC-P, ELISA - Q96N28

Core Product Specifications and Parameters

Biological Background: PRELID3A Function and Localization

• PRELID3A, also known as SLMO1, is a PRELI domain-containing protein encoded by the PRELID3A gene (formerly C18orf43) on chromosome 18.
• In vitro, the TRIAP1:PRELID3A complex mediates the transfer of phosphatidic acid (PA) between liposomes, functioning as a PA transporter across the mitochondrial intermembrane space.
• Phosphatidic acid import by this complex is required for cardiolipin (CL) synthesis in the mitochondrial inner membrane, a critical lipid for mitochondrial function.
• PRELID3A localizes specifically to the mitochondrion, as annotated in UniProt.
• The protein undergoes alternative splicing, generating distinct isoforms with potentially different functional properties.
• Additional keywords from UniProt include "Lipid transport", "Transport", and "3D-structure", reflecting its role in mitochondrial lipid trafficking.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide spanning amino acids 70–172 (NP_006544.2). When validating, consider using the peptide as a competitive control in ELISA or blocking experiments.
• For IHC-P, optimize antigen retrieval conditions appropriate for mitochondrial targets in human tissue sections. Validate with appropriate positive and negative control tissues.
• Since the antibody is polyclonal, it may detect multiple isoforms resulting from alternative splicing. Confirm specificity via Western blot if needed, though not validated by supplier.
• In ELISA, the antibody may be used for direct or indirect detection of PRELID3A in human samples; consider pairing with a secondary detection system optimized for rabbit antibodies.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research labs with streamlined access to a broad portfolio of IVD raw materials, including validated antibody pairs, optimized polyclonal and monoclonal antibodies, and bulk ancillary reagents. For mitochondrial biology and lipid metabolism targets like PRELID3A, we offer reliable sourcing solutions to support cardiolipin research and related applications. Beyond individual products, we help partners scale from early-stage development to clinical readiness through integrated technical and supply chain services.