Anti-PRELID1 Polyclonal Antibody for IHC-P, IF/ICC, ELISA - Q9Y255

Core Product Specifications and Parameters

Biological Background: PRELID1 Function and Localization

• PRELID1 (PRELI domain-containing protein 1, also known as PX19, PRELI, SBBI12, CGI-106) is a 25 kDa mitochondrial intermembrane space protein.
• It plays a critical role in the mitochondrial apoptotic pathway by ensuring the accumulation of cardiolipin (CL) in mitochondrial membranes.
• Forms a complex with TRIAP1 that transfers phosphatidic acid (PA) between liposomes, likely acting as a PA transporter across the intermembrane space for CL synthesis in the inner membrane.
• Modulates T helper cell differentiation by down-regulating STAT6, thereby reducing IL-4-induced Th2 cell numbers.
• Highly expressed in fetal liver and, in adults, predominantly in spleen, brain, lung, lymph nodes, and peripheral blood leukocytes; expression is drastically reduced in adult liver.
• Localizes to the mitochondrion, specifically the intermembrane space, with a transit peptide directing mitochondrial import.
• Keywords associated with PRELID1 include apoptosis, lipid transport, alternative splicing, and mitochondrial function.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 174–219 of human PRELID1; consider sequence alignment to assess cross-reactivity with homologs or isoforms.
• For IHC-P, standard antigen retrieval (e.g., citrate buffer pH 6.0) and titration may be required to optimize specific staining.
• For IF/ICC, ensure mitochondrial staining pattern; permeabilization with Triton X-100 or digitonin may be necessary; validate co-localization with mitochondrial markers.
• ELISA applications may be used for detecting recombinant or native PRELID1; standard indirect ELISA protocols should be validated with appropriate controls.
• Expected molecular weight is ~25 kDa; additional bands may correspond to post-translational modifications or isoforms (alternative splicing reported).

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research institutes with one-stop access to high-quality IVD raw materials, including validated antibody pairs, optimized polyclonal antibodies like this PRELID1 antibody, and bulk ancillary reagents. Whether your work focuses on mitochondrial biology, apoptosis, or immunological assays, we streamline sourcing from concept to clinic. Our supply capabilities cover rare-target raw materials and technical consultation to support your assay development.