Anti-PREB Rabbit Monoclonal Antibody for WB, IF/ICC, ELISA - Q9HCU5

Core Product Specifications and Parameters

Biological Background: PREB Function and Localization

• PREB functions as a guanine nucleotide exchange factor (GEF) that selectively activates SAR1A and SAR1B, triggering COPII coat assembly and ER-to-Golgi vesicle transport. Related references: PMID:32358066

• It also binds the prolactin (PRL) gene promoter and may act as a transcription regulator.

• The protein localizes to both the endoplasmic reticulum membrane and the nucleus.

• PREB is ubiquitously expressed across human tissues.

• It has a predicted molecular weight of ~45 kDa and contains WD repeats; it may undergo nitration.

Experimental Guidance and Technical Tips

• The immunogen spans residues 89–201 of human PREB; verify reactivity in your target species and optimize dilutions for each application.
• For Western blot, expect a band at ~45 kDa; include appropriate loading controls and validate with positive controls from human, mouse, or rat lysates.
• For immunofluorescence/ICC, permeabilization may be required to detect nuclear and ER membrane pools; use species-matched secondary antibodies.
• This antibody has been reported to react with human, mouse, and rat samples; cross-reactivity with other species should be tested empirically.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a dedicated IVD raw-material partner, offering validated monoclonal/polyclonal antibodies, antibody pairs, and bulk ancillary reagents to support your assay development. This PREB antibody targets a key regulator of ER-Golgi transport and transcription, making it suitable for cell biology, cancer, or endocrinology research. For rare targets or customized sourcing needs, CamelBio provides one‑stop access from concept to clinic, ensuring you get the right materials for your diagnostic workflows.