Anti-PRDM7 Polyclonal Antibody for WB, IF/ICC, ELISA - Q9NQW5

Core Product Specifications and Parameters

Biological Background: PRDM7 Function and Localization

• PRDM7 is a histone-lysine N-methyltransferase that selectively converts dimethylated lysine 4 on histone H3 (H3K4me2) to trimethylated H3K4 (H3K4me3), an epigenetic mark linked to active chromatin and transcriptional activation.
• Its methyltransferase activity is S-adenosyl-L-methionine (SAM)-dependent, a hallmark of SET domain-containing enzymes.
• PRDM7 belongs to the PRDM (PRDI-BF1 and RIZ homology domain) protein family, members of which typically contain an N-terminal PR domain and multiple C-terminal C2H2 zinc finger motifs, suggesting roles in both chromatin modification and DNA binding.
• The protein localizes to the nucleus and associates with chromosomes, aligning with its function as a chromatin regulator.
• Alternative splicing of the PRDM7 gene may yield multiple isoforms, as indicated by UniProt, potentially expanding its functional diversity.
• As a chromatin regulator and methyltransferase, PRDM7 participates in epigenetic control of gene expression, likely by establishing permissive chromatin states at target genomic loci.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a central region (amino acids 210–310) of human PRDM7, which may include part of the zinc finger domain; this design likely provides specificity for PRDM7 over closely related PRDM family members, but cross-reactivity should be verified in the relevant sample system.
• For Western blot (WB), the predicted molecular weight is approximately 56 kDa; however, due to potential post‑translational modifications or alternative splicing, additional bands or slight shifts may be observed—validate using appropriate controls.
• In immunofluorescence (IF/ICC), expect nuclear staining; optimize fixation (e.g., methanol or paraformaldehyde) and permeabilization conditions to ensure proper antibody access to chromatin-associated epitopes.
• For ELISA, the antibody can be used for titer determination or screening applications; always determine the optimal working dilution empirically for your specific experimental setup.
• When combining with other antibodies (e.g., in multiplexed IF or co-IP), confirm compatibility of host species and conjugate labels to avoid interference.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive procurement partner for IVD raw materials, supporting your epigenetic research with validated antibody pairs, recombinant proteins, and ancillary reagents. For PRDM7-focused projects, we offer bulk quantities of polyclonal antibodies, monoclonal alternatives, and custom development options to streamline your assay development. Our sourcing capabilities extend to rare targets and specialized reagents, ensuring a reliable supply chain from concept to clinic.