Anti-PRDM6 Polyclonal Antibody for WB, ELISA - Q9NQX0

Core Product Specifications and Parameters

Biological Background: PRDM6 Function and Localization

• PRDM6 (PR domain zinc finger protein 6), also known as PDA3, KMT8C, or PRISM, is a member of the PR/SET domain family of putative histone methyltransferases.
• It functions primarily as a transcriptional repressor, particularly of smooth muscle gene expression, helping to maintain the proliferative potential of vascular smooth muscle cells.
• In endothelial cells, PRDM6 inhibits proliferation, survival, and differentiation, suggesting a role in vascular development and pathology.
• PRDM6 is localized exclusively to the nucleus, consistent with its function as a chromatin regulator and transcription factor.
• The protein contains multiple zinc-finger repeats and a PR/SET domain, which may mediate protein-protein interactions and potential histone methyltransferase activity, though its enzymatic activity in vivo remains unclear.
• PRDM6 has been implicated in recruiting the histone methyltransferase EHMT2/G9a to target genes, thereby facilitating H3K9 methylation and gene silencing.
• Alternative splicing of the PRDM6 gene produces multiple isoforms, which may have distinct tissue-specific or functional roles.
• According to some in vitro studies, PRDM6 can specifically methylate lysine 20 of histone H4 (H4K20), a modification associated with transcriptional repression.

Experimental Guidance and Technical Tips

• For Western blotting, a band of approximately 64 kDa corresponding to PRDM6 is expected in lysates from human, mouse, and rat cells.
• Due to potential isoform variation, multiple bands may be observed; validate in the relevant sample system using positive and negative controls.
• The immunogen spans amino acids 376–595, a region that may be conserved across species, supporting the predicted cross-reactivity.
• For ELISA, consider using the antibody as a capture or detection reagent after confirming performance with recombinant PRDM6 protein.
• Always optimize antibody dilution and incubation conditions based on your specific experimental setup.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your dedicated partner for IVD raw material supply, offering a one-stop solution from concept to clinic. This anti-PRDM6 polyclonal antibody supports research into chromatin regulation, smooth muscle biology, and vascular disease mechanisms. In addition to this product, we can source validated antibody pairs, bulk monoclonal/polyclonal antibodies, and ancillary reagents to accelerate your assay development.