Anti-PRDM2 Rabbit Polyclonal Antibody for WB and ELISA - Q13029

Core Product Specifications and Parameters

Biological Background: PRDM2 Function and Localization

• PRDM2 (PR domain zinc finger protein 2), also known as RIZ, KMT8, MTB-ZF, or retinoblastoma protein-interacting zinc finger protein, is a member of the PR/SET domain family and functions as a histone lysine methyltransferase.
• It specifically catalyzes the S-adenosyl-L-methionine-dependent monomethylation of histone H3 at lysine 9 (H3K9me1), an epigenetic mark linked to transcriptional regulation.
• Beyond its catalytic role, PRDM2 may function as a DNA-binding transcription factor; it binds the macrophage-specific TPA-responsive element (MTE) of the heme oxygenase 1 (HMOX1) gene and potentially activates its transcription.
• PRDM2 is localized to the nucleus, consistent with its roles in chromatin modification and transcriptional control.
• Tissue expression is broad but notably high in retinoblastoma cell lines and brain tumors, with isoform 1 (RIZ1) showing significantly elevated expression in testis compared to isoform 3 (RIZ2).
• The protein contains multiple zinc-finger motifs and a PR domain, and is subject to post-translational modifications including phosphorylation and ubiquitination (isopeptide bond formation).

Experimental Guidance and Technical Tips

• The immunogen design targets the N-terminal region (aa 1-176), which may recognize both major isoforms (RIZ1 and RIZ2) if this region is conserved; verify isoform specificity in your sample system.
• For Western blot, consider using nuclear extracts from human, mouse, or rat tissues, and confirm the expected band around 189 kDa with appropriate molecular weight markers.
• When performing ELISA, pairing this polyclonal antibody with a suitable detection system may enhance signal; titrate the antibody to determine optimal working concentration.
• Given the reported expression in retinoblastoma and brain tumor samples, ensure relevant positive and negative controls are included to validate specificity in cancer-related studies.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers and research laboratories with comprehensive access to IVD raw materials, including validated antibody pairs and optimized polyclonal antibodies like this anti-PRDM2 reagent. For projects requiring epigenetic or transcriptional targets, we offer bulk ancillary reagents and rare target sourcing to streamline your workflow from discovery to clinical application. Contact us to discuss customized procurement solutions tailored to your PRDM2-related research needs.