Anti-PRDM14 Rabbit Polyclonal Antibody for WB, IHC-P, ELISA, ChIP - Q9GZV8

Core Product Specifications and Parameters

Biological Background: PRDM14 Function and Localization

• PRDM14 (PR domain zinc finger protein 14) is a transcription factor that acts as both activator and repressor, crucial for maintaining embryonic stem cell identity and reacquiring pluripotency in somatic cells.
• It plays a dual role in germ cell development: reacquisition of potential pluripotency (including SOX2 up-regulation) and successful epigenetic reprogramming via EHMT1 repression.
• The interaction with CBFA2T2 is necessary for its functions in pluripotency and germ cell formation.
• PRDM14 directly up-regulates POU5F1 (OCT4) by binding to its proximal enhancer at the consensus sequence 5'-GGTC[TC]CTAA-3'.
• Subcellular localization is predominantly nuclear, consistent with its DNA-binding transcription factor role.
• Tissue-specific expression is high in embryonic stem cells, and at protein level, it tends to be overexpressed in breast cancer.
• Key structural features include a PR domain with potential methyltransferase activity, multiple zinc fingers, and phosphorylation sites, collectively mediating its regulatory functions.

Experimental Guidance and Technical Tips

• The immunogen used is a recombinant fusion protein corresponding to amino acids 1-260 of human PRDM14, which may recognize both full-length and potential N-terminal isoforms.
• For Western blotting, a band at approximately 64 kDa is expected; validate using positive controls such as embryonic stem cell lysates.
• For IHC-P, consider optimizing antigen retrieval methods and validating staining with appropriate tissue sections (e.g., embryonic stem cell clusters, breast cancer tissues).
• For ChIP assays, verify antibody specificity and confirm the enrichment of known PRDM14 target sequences, such as the POU5F1 proximal enhancer.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, research institutes, and laboratories with one-stop access to high-quality IVD raw materials for stem cell and developmental biology research. Complement your PRDM14-related studies with our validated antibody pairs, optimized polyclonal antibodies, and ancillary reagents. For rare or bulk raw-material sourcing needs, CamelBio’s comprehensive supply chain can bridge your requirements from concept to clinic.